Discovery and characterization of single nucleotide polymorphisms in two anadromous alosine fishes of conservation concern

Details

• Alternative Title:
  Discovery and characterization of single nucleotide polymorphisms in two anadromous alosine fishes of conservation concern
• Journal Title:
  Ecology and Evolution
• Personal Author:
  Baetscher, Diana S. ; Hasselman, Daniel J. ; Reid, Kerry ; Palkovacs, Eric P. ; Garza, John Carlos
• NOAA Program & Office:
  NMFS (National Marine Fisheries Service) ; SWFSC (Southwest Fisheries Science Center) ; CIMEC (Cooperative Institute for Marine Ecosystems and Climate)
• Description:
  Freshwater habitat alteration and marine fisheries can affect anadromous fish species, and populations fluctuating in size elicit conservation concern and coordinated management. We describe the development and characterization of two sets of 96 single nucleotide polymorphism (SNP) assays for two species of anadromous alosine fishes, alewife and blueback herring (collectively known as river herring), that are native to the Atlantic coast of North America. We used data from high-throughput DNA sequencing to discover SNPs and then developed molecular genetic assays for genotyping sets of 96 individual loci in each species. The two sets of assays were validated with multiple populations that encompass both the geographic range and the known regional genetic stocks of both species. The SNP panels developed herein accurately resolved the genetic stock structure for alewife and blueback herring that was previously identified using microsatellites and assigned individuals to regional stock of origin with high accuracy. These genetic markers, which generate data that are easily shared and combined, will greatly facilitate ongoing conservation and management of river herring including genetic assignment of marine caught individuals to stock of origin.
• Source:
  Ecology and Evolution
• DOI:
  https://doi.org/10.1002/ece3.3215
• Pubmed Central ID:
  PMC5587496
• Document Type:
  Journal Article
• Place as Subject:
  Canada
• Rights Information:
  CC BY
• Compliance:
  PMC
• Download URL:
  https://repository.library.noaa.gov/view/noaa/25999/noaa_25999_DS1.pdf
• File Type:
  [PDF - 583.71 KB]
• Collection(s):
  NOAA Cooperative Institutes ; National Marine Fisheries Service (NMFS)
• Main Document Checksum:
  urn:sha-512:16f364c9b1de3db044b9fdaeeafd3616fcd2ff6a089954ba2be36604d7b00bf0235d7cf9e082b708ce502ae1c9ed74ad37999b1631b8b4aa3bed02cf11a5d24b