Accuracy enhancement of microscope enumeration of picoplankter Aureococcus anophagefferens

Mahoney, John B.; Jeffress, Dorothy; Bredemeyer, John; Wendling, Kari

i

Accuracy enhancement of microscope enumeration of picoplankter Aureococcus anophagefferens

Supporting Files

2003
By Mahoney, John B. ; Jeffress, Dorothy ; Bredemeyer, John
Series: Northeast Fisheries Science Center reference document ; 03-11

Details

Personal Author:

Mahoney, John B. ; Jeffress, Dorothy ; Bredemeyer, John ; Wendling, Kari
Corporate Authors:

Northeast Fisheries Science Center (U.S.)
NOAA Program & Office:

NMFS (National Marine Fisheries Service) ; NEFSC (Northeast Fisheries Science Center)
Description:

Two main types of enumeration error were experienced in the use of an immunofluorescence protocol for identification of the picoplankter Aureococcus anophagefferens from western New York Bight coastal waters. Microscopist discrimination was affected when cells were too numerous to count reliably or too lightly stained to be identified. This was remedied by sample dilution, and increase of antisera and stain concentration and incubation time. The second type, extensive clustering of A. anophagefferens cells in monospecific clumps, and as embeds in floc matrices, was much more problematic. To remedy this, cell disaggregation was sought through mechanical and chemical treatments. A single treatment effective for all samples was not found but various treatment combinations greatly improved enumeration. Varied efficacy of treatment regimes among samples suggests that cell binding strength was variable.
Content Notes:

by John B. Mahoney, Dorothy Jeffress, John Bredemeyer, and Kari Wendling ; "August 2003." ; "Web version posted August 29, 2003." ; System requirements: Adobe Acrobat Reader. ; Includes bibliographical references (pages 14-15).
Series:

Northeast Fisheries Science Center reference document ; 03-11
Document Type:

Miscellaneous
Place as Subject:

New York Bight (N.J. and N.Y.) ; Atlantic Coast (U.S.)
Rights Information:

Public Domain
Compliance:

Library
Download URL:

https://repository.library.noaa.gov/view/noaa/5343/noaa_5343_DS1.pdf
File Type:

[PDF - 579.24 KB]
Collection(s):

National Marine Fisheries Service (NMFS)
Main Document Checksum:

urn:sha256:aa44de47111689f481e690ac4df133b94e7efd36d86c10128735159b58a09401

Document's home page

html

ON THIS PAGE

Details Supporting Files

Found an issue?

Send us an email at:

publishinghd@cdc.gov

The NOAA IR serves as an archival repository of NOAA-published products including scientific findings, journal articles, guidelines, recommendations, or other information authored or co-authored by NOAA or funded partners. As a repository, the NOAA IR retains documents in their original published format to ensure public access to scientific information.