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Species-specific PCR assays for Gambierdiscus excentricus and Gambierdiscus silvae (Gonyaulacales, Dinophyceae)
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2019
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Source: J. Phycol., 55: 730-732
[PDF-260.87 KB]
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Journal Title:Journal of Phycology
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Description:The two most toxic Gambierdiscus species identified from the Caribbean are G. excentricus and G. silvae. These species are the primary causes of ciguatera fish poisoning and likely contribute disproportionately to the toxicity of marine food webs. While Gambierdiscus species are difficult to distinguish using light or scanning electron microscopy, reliable species-specific molecular identification methods have been developed and used successfully to identify a number of other Gambierdiscus species. Corresponding species-specific assays are not yet available for G. excentricus and G. silvae, which imposes limitations on species identification and related ecological studies. The following note describes species-specific polymerase chain reaction assays for G. excentricus and G. silvae that can be used for these purposes.
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Content Notes:This project was supported by the NOAA Fisheries National Seabird Program and the Volgenau Foundation. AR acknowledges support from the National Oceanic and Atmospheric Administration Dr. Nancy Foster Scholarship Program.
We are indebted to the observers of the Northeast Fisheries Observer Program and Stephanie DePasquale for their assistance obtaining samples used in this study. We also thank Maelle Connan (Nelson Mandela University) for assistance procuring samples. Although NOAA employees contributed to this article, this research was conceived, designed, and implemented by URI.
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Source:J. Phycol., 55: 730-732
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