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A qPCR-Based Survey of Haplosporidium nelsoni and Perkinsus spp. in the Eastern Oyster, Crassostrea virginica in Maine, USA

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Details

  • Journal Title:
    Pathogens
  • Personal Author:
  • NOAA Program & Office:
    NMFS (National Marine Fisheries Service)
  • Description:
    Eastern oyster (Crassostrea virginica) aquaculture is increasingly playing a significant role in the state of Maine's (USA) coastal economy. Here, we conducted a qPCR-based survey for Haplosporidium nelsoni, Perkinsus marinus, and Perkinsus chesapeaki in C. virginica (n = 1440) from six Maine sites during the summer-fall of 2016 and 2017. In the absence of reported die-offs, our results indicated the continued presence of the three protozoan parasites in the six sites. The highest H. nelsoni qPCR-prevalence corresponded to Jack's Point and Prentiss Island (x over bar = 40 and 48% respectively), both located in the Damariscotta River Estuary. Jack's Point, Prentiss Island, New Meadows River, and Weskeag River recorded the highest qPCR-prevalence for P. marinus (32-39%). While the P. marinus qPCR-prevalence differed slightly for the years 2016 and 2017, P. chesapeaki qPCR-prevalence in 2016 was markedly lower than 2017 (60% at all sites for each of the years, respectively). Mean qPCR-prevalence values for P. chesapeaki over the two-year study were >= 40% for samples from Jack's Point (49%), Prentiss Island (44%), and New Meadows River (40%). This study highlights that large and sustained surveys for parasitic diseases are fundamental for decision making toward the management of the shellfish aquaculture industry, especially for having a baseline in the case that die-offs occur.
  • Keywords:
  • Source:
    Pathogens, 9(4), 256
  • DOI:
  • Document Type:
    Journal Article
  • Funding:
    Grant no. NA15NMF4270303
  • Place as Subject:
    Maine
  • License:
  • Rights Information:
    CC BY
  • Compliance:
    Library
  • Main Document Checksum:
    urn:sha256:c1713f0c7fa7d5e3ea947240ee5c31bf4ae3b09f3e14b10cd81997ef3ae38004
  • Download URL:
  • File Type:
    Filetype[PDF - 2.88 MB ]
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