Details:

Journal Title:
Molecular and Cellular Probes
Personal Author:
Dickerson, J. ; Gooch-Moore, J. ; Jacobs, J.M. ; Mott, J.B.
Dickerson, J. ; Gooch-Moore, J. ; Jacobs, J.M. ; Mott, J.B. Less -
NOAA Program & Office:
NOS (National Ocean Service) ; NCCOS (National Centers for Coastal Ocean Science)
NOS (National Ocean Service) ; NCCOS (National Centers for Coastal Ocean Science) Less -
Description:
Researchers have developed multiple methods to characterize clinical and environmental strains of Vibrio vulnificus. The aim of our study was to use four assays to detect virulence factors in strains from infected patients and those from surface waters/sediments/oysters of South Carolina and the Gulf of Mexico. Vibrio vulnificus strains from clinical (n = 81) and environmental (n = 171) sources were tested using three real-time PCR methods designed to detect polymorphisms in the 16S rRNA, vcg and pilF genes and a phenotypic method, the ability to ferment D-mannitol. Although none of the tests correctly categorized all isolates, the differentiation between clinical and environmental isolates was similar for the pilF, vcgC/E and 16S rRNA assays, with sensitivities of 74.1–79.2% and specificities of 77.4–82.7%. The pilF and vcgC/E assays are comparable in efficacy to the widely used 16S rRNA method, while the D-mannitol fermentation test is less discriminatory (sensitivity = 77.8%, specificity = 61.4%). Overall percent agreement for the D-mannitol fermentation method was also lower (66.7%) than overall percent agreement for the 3 molecular assays (78.0%–80.2%). This study demonstrated, using a large, diverse group of Vibrio vulnificus isolates, that three assays could be used to distinguish most clinical vs environmental isolates; however, additional assays are needed to increase accuracy.
Keywords:
[+]
Genotyping Vibrio Vulnificus
Source:
Molecular and Cellular Probes, 56, 101695
DOI:
https://doi.org/10.1016/j.mcp.2021.101695
ISSN:
0890-8508
Publisher:
Elsevier BV
Document Type:
Journal Article
Funding:
Grant no. NA08NOS4730440
Rights Information:
Accepted Manuscript
Rights Statement:
This manuscript is made available under the Elsevier user license https://www.elsevier.com/open-access/userlicense/1.0/
Compliance:
Library
Main Document Checksum:
[+]
urn:sha256:14835d252ae2eb921cf97380abb12d18c16f42fdddd1e023daf606196e862d4d
Download URL:
https://repository.library.noaa.gov/view/noaa/50832/noaa_50832_DS1.pdf
File Type:

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