Comparative Study on the Performance of Three Detection Methods for the Quantification of Pacific Ciguatoxins in French Polynesian Strains of Gambierdiscus polynesiensis

Darius, Hélène Taiana; Revel, Taina; Viallon, Jérôme; Sibat, Manoëlla; Cruchet, Philippe; Longo, Sébastien; Hardison, Donnie Ransom; Holland, William C.; Tester, Patricia A.; Litaker, R. Wayne; McCall, Jennifer R.; Hess, Philipp; Chinain, Mireille

doi:10.3390/md20060348

i

Comparative Study on the Performance of Three Detection Methods for the Quantification of Pacific Ciguatoxins in French Polynesian Strains of Gambierdiscus polynesiensis

Supporting Files

2022
By Darius, Hélène Taiana ; Revel, Taina ; Viallon, Jérôme ; ...

Details

Journal Title:

Marine Drugs
Personal Author:

Darius, Hélène Taiana ; Revel, Taina ; Viallon, Jérôme ; Sibat, Manoëlla ; Cruchet, Philippe ; Longo, Sébastien ; Hardison, Donnie Ransom ; Holland, William C. ; Tester, Patricia A. ; Litaker, R. Wayne ; McCall, Jennifer R. ; Hess, Philipp ; Chinain, Mireille
NOAA Program & Office:

NOS (National Ocean Service) ; NCCOS (National Centers for Coastal Ocean Science)
Description:

Gambierdiscus and Fukuyoa dinoflagellates produce a suite of secondary metabolites, including ciguatoxins (CTXs), which bioaccumulate and are further biotransformed in fish and marine invertebrates, causing ciguatera poisoning when consumed by humans. This study is the first to compare the performance of the fluorescent receptor binding assay (fRBA), neuroblastoma cell-based assay (CBA-N2a), and liquid chromatography tandem mass spectrometry (LC-MS/MS) for the quantitative estimation of CTX contents in 30 samples, obtained from four French Polynesian strains of Gambierdiscus polynesiensis. fRBA was applied to Gambierdiscus matrix for the first time, and several parameters of the fRBA protocol were refined. Following liquid/liquid partitioning to separate CTXs from other algal compounds, the variability of CTX contents was estimated using these three methods in three independent experiments. All three assays were significantly correlated with each other, with the highest correlation coefficient (r2 = 0.841) found between fRBA and LC-MS/MS. The CBA-N2a was more sensitive than LC-MS/MS and fRBA, with all assays showing good repeatability. The combined use of fRBA and/or CBA-N2a for screening purposes and LC-MS/MS for confirmation purposes allows for efficient CTX evaluation in Gambierdiscus. These findings, which support future collaborative studies for the inter-laboratory validation of CTX detection methods, will help improve ciguatera risk assessment and management.
Keywords:

Ciguatoxins Gambierdiscus Polynesiensis Gambierdiscus Polynesiensis
Source:

Marine Drugs, 20(6), 348
DOI:

https://doi.org/10.3390/md20060348
ISSN:

1660-3397
Publisher:

MDPI AG
Document Type:

Journal Article
License:

https://creativecommons.org/licenses/by/4.0/
Rights Information:

CC BY
Compliance:

Library
Main Document Checksum:

urn:sha256:635b8df24135c6cf2b8c00a954bea06776a0bf5489f5137a899661a662cbd79d
Download URL:

https://repository.library.noaa.gov/view/noaa/50754/noaa_50754_DS1.pdf
File Type:

[PDF - 3.44 MB ]

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