Molecular Evidence For Sediment Nitrogen Fixation In A Temperate New England Estuary

Newell, Silvia E.; Pritchard, Kaitlyn R.; Foster, Sarah Q.; Fulweiler, Robinson W.

doi:10.7717/peerj.1615

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i

Molecular Evidence For Sediment Nitrogen Fixation In A Temperate New England Estuary

2016
By Newell, Silvia E. ; Pritchard, Kaitlyn R. ; Foster, Sarah Q. ; ...
Source: PeerJ 4:e1615

[PDF-2.53 MB]

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Journal Title:

PeerJ
Personal Author:

Newell, Silvia E. ; Pritchard, Kaitlyn R. ; Foster, Sarah Q. ; Fulweiler, Robinson W.

Newell, Silvia E. ; Pritchard, Kaitlyn R. ; Foster, Sarah Q. ; Fulweiler, Robinson W. Less -
NOAA Program & Office:

OAR (Oceanic and Atmospheric Research) ; Sea Grant

OAR (Oceanic and Atmospheric Research) ; Sea Grant Less -
Sea Grant Program:

WHOI (Woods Hole Sea Grant) ; MIT (MIT Sea Grant)

WHOI (Woods Hole Sea Grant) ; MIT (MIT Sea Grant) Less -
Description:

Primary production in coastal waters is generally nitrogen (N) limited with denitrification outpacing nitrogen fixation (N2-fixation). However, recent work suggests that we have potentially underestimated the importance of heterotrophic sediment N2-fixation in marine ecosystems. We used clone libraries to examine transcript diversity of nifH (a gene associated with N2-fixation) in sediments at three sites in a temperate New England estuary (Waquoit Bay, Massachusetts, USA) and compared our results to net sediment N2 fluxes previously measured at these sites. We observed nifH expression at all sites, including a site heavily impacted by anthropogenic N. At this N impacted site, we also observed mean net sediment N2-fixation, linking the geochemical rate measurement with nifH expression. This same site also had the lowest diversity (non-parametric Shannon = 2.75). At the two other sites, we also detected nifH transcripts, however, the mean N2 flux indicated net denitrification. These results suggest that N2-fixation and denitrification co-occur in these sediments. Of the unique sequences in this study, 67% were most closely related to uncultured bacteria from various marine environments, 17% to Cluster III, 15% to Cluster I, and only 1% to Cluster II. These data add to the growing body of literature that sediment heterotrophic N2-fixation, even under high inorganic nitrogen concentrations, may be an important yet overlooked source of N in coastal systems.
Keywords:

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Estuaries Sediments
Source:

PeerJ 4:e1615
DOI:

https://doi.org/10.7717/peerj.1615
Sea Grant Document Number:

WHOI-R-16-001
Document Type:

Journal Article
Place as Subject:

New England
Rights Information:

CC BY
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urn:sha256:e4f92d3227425ab25a38005f30c548680ccc18548d1a89256facef43c3acb028
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https://repository.library.noaa.gov/view/noaa/40031/noaa_40031_DS1.pdf
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Molecular Evidence For Sediment Nitrogen Fixation In A Temperate New England Estuary

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