Sponging up diversity: Evaluating metabarcoding performance for a taxonomically challenging phylum within a complex cryptobenthic community

Timmers, Molly A.; Vicente, Jan; Webb, Maryann; Jury, Christopher P.; Toonen, Robert J.

doi:10.1002/edn3.163

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Sponging up diversity: Evaluating metabarcoding performance for a taxonomically challenging phylum within a complex cryptobenthic community

2020
By Timmers, Molly A. ; Vicente, Jan ; Webb, Maryann ; ...
Source: Environmental DNA, 00

[PDF-1.07 MB]

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Details:

Journal Title:

Environmental DNA
Personal Author:

Timmers, Molly A. ; Vicente, Jan ; Webb, Maryann ; Jury, Christopher P. ; Toonen, Robert J.

Timmers, Molly A. ; Vicente, Jan ; Webb, Maryann ; Jury, Christopher P. ; Toonen, Robert J. Less -
NOAA Program & Office:

NMFS (National Marine Fisheries Service) ; PIFSC (Pacific Islands Fisheries Science Center) ; JIMAR (Joint Institute for Marine and Atmospheric Research)

NMFS (National Marine Fisheries Service) ; PIFSC (Pacific Islands Fisheries Science Center) ; JIMAR (Joint Institute for Marine and Atmospheric Research) Less -
Description:

Despite their ecological importance, sponges are often avoided in biodiversity studies and monitoring programs because they are notoriously difficult to identify using morphological or molecular methods. Here, we investigate the metabarcoding performance of universal degenerate cytochrome c oxidase subunit I (COI) primers in detecting species from this challenging phylum in a cryptobenthic community.
Keywords:

[+]

28S, COI, Community metabarcoding, Coral reefs, Cryptobenthic, DNA barcoding, Mesocosm, Porifera
Source:

Environmental DNA, 00
DOI:

https://doi.org/10.1002/edn3.163
Document Type:

Journal Article
Funding:

Grant no. NA16NMF4320058
Rights Information:

CC BY
Compliance:

Submitted
Main Document Checksum:

[+]

urn:sha256:ae2533753dfde6243e1288652e2322186f99749f380378585f49227fd5a9356a
Download URL:

https://repository.library.noaa.gov/view/noaa/32422/noaa_32422_DS1.pdf
File Type:

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