Toxicological Investigations on the Sea Urchin Tripneustes gratilla (Toxopneustidae, Echinoid) from Anaho Bay (Nuku Hiva, French Polynesia): Evidence for the Presence of Pacific Ciguatoxins

Darius, Hélène Taiana; Roué, Mélanie; Sibat, Manoella; Viallon, Jérôme; Gatti, Clémence Mahana iti; Vandersea, Mark W.; Tester, Patricia A.; Litaker, R. Wayne; Amzil, Zouher; Hess, Philipp; Chinain, Mireille

doi:10.3390/md16040122

i

Toxicological Investigations on the Sea Urchin Tripneustes gratilla (Toxopneustidae, Echinoid) from Anaho Bay (Nuku Hiva, French Polynesia): Evidence for the Presence of Pacific Ciguatoxins

2018
By Darius, Hélène Taiana ; Roué, Mélanie ; Sibat, Manoella ; ...

Details

Journal Title:

Marine Drugs
Personal Author:

Darius, Hélène Taiana ; Roué, Mélanie ; Sibat, Manoella ; Viallon, Jérôme ; Gatti, Clémence Mahana iti ; Vandersea, Mark W. ; Tester, Patricia A. ; Litaker, R. Wayne ; Amzil, Zouher ; Hess, Philipp ; Chinain, Mireille
NOAA Program & Office:

NOS (National Ocean Service) ; NCCOS (National Centers for Coastal Ocean Science)
Description:

The sea urchin Tripneustes gratilla (Toxopneustidae, Echinoids) is a source of protein for many islanders in the Indo-West Pacific. It was previously reported to occasionally cause ciguatera-like poisoning; however, the exact nature of the causative agent was not confirmed. In April and July 2015, ciguatera poisonings were reported following the consumption of T. gratilla in Anaho Bay (Nuku Hiva Island, Marquesas archipelago, French Polynesia). Patient symptomatology was recorded and sea urchin samples were collected from Anaho Bay in July 2015 and November 2016. Toxicity analysis using the neuroblastoma cell–based assay (CBA-N2a) detected the presence of ciguatoxins (CTXs) in T. gratilla samples. Gambierdiscus species were predominant in the benthic assemblages of Anaho Bay, and G. polynesiensis was highly prevalent in in vitro cultures according to qPCR results. Liquid chromatography–tandem mass spectrometry (LC-MS/MS) analyses revealed that P-CTX-3B was the major ciguatoxin congener in toxic sea urchin samples, followed by 51-OH-P-CTX-3C, P-CTX-3C, P-CTX-4A, and P-CTX-4B. Between July 2015 and November 2016, the toxin content in T. gratilla decreased, but was consistently above the safety limit allowed for human consumption. This study provides evidence of CTX bioaccumulation in T. gratilla as a cause of ciguatera-like poisoning associated with a documented symptomatology.
Keywords:

Poisonous Fishes Research Sea Urchins Toxicity Testing Toxicology Poisonous Fishes Sea Urchins Toxicity Testing
Source:

Mar. Drugs; 16(4) 1-22
DOI:

https://doi.org/10.3390/md16040122
Pubmed Central ID:

PMC5923409
Document Type:

Journal Article
Place as Subject:

Nuka Hiva (French Polynesia)
Rights Information:

CC BY
Compliance:

PMC
Main Document Checksum:

urn:sha-512:fb8eb702691c033c7a067ff1e18ba1efd77f755d661f9d5fbb459a64ae346694ae43c74968577b66b75fc7a83bc1584b784cb606a0d68a07fb858887d085b3c6
Download URL:

https://repository.library.noaa.gov/view/noaa/25615/noaa_25615_DS1.pdf
File Type:

[PDF - 5.46 MB ]

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