Cell cycle arrest and biochemical changes accompanying cell death in harmful dinoflagellates following exposure to bacterial algicide IRI-160AA

Pokrzywinski, Kaytee L.; Tilney, Charles L.; Warner, Mark E.; Coyne, Kathryn J.

doi:10.1038/srep45102

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i

Cell cycle arrest and biochemical changes accompanying cell death in harmful dinoflagellates following exposure to bacterial algicide IRI-160AA

2017
By Pokrzywinski, Kaytee L. ; Tilney, Charles L. ; Warner, Mark E. ; ...
Source: Scientific Reports 7 (45102)

[PDF-1.43 MB]

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Journal Title:

Scientific Reports
Personal Author:

Pokrzywinski, Kaytee L. ; Tilney, Charles L. ; Warner, Mark E. ; Coyne, Kathryn J.

Pokrzywinski, Kaytee L. ; Tilney, Charles L. ; Warner, Mark E. ; Coyne, Kathryn J. Less -
NOAA Program & Office:

NOS (National Ocean Service) ; NCCOS (National Centers for Coastal Ocean Science)

NOS (National Ocean Service) ; NCCOS (National Centers for Coastal Ocean Science) Less -
Description:

Bacteria may play a role in regulating harmful algal blooms, but little is known about the biochemical and physiological changes associated with cell death induced by algicidal bacteria. Previous work characterized an algicidal exudate (IRI-160AA) produced by Shewanella sp. IRI-160 that is effective against dinoflagellates, while having little to no effect on other phytoplankton species in laboratory culture experiments. The objective of this study was to evaluate biochemical changes associated with cell death and impacts on the cell cycle in three dinoflagellate species (Prorocentrum minimum, Karlodinium veneficum and Gyrodinium instriatum) after exposure to IRI-160AA. In this study, IRI-160AA induced cell cycle arrest in all dinoflagellates examined. Several indicators for programmed cell death (PCD) that are often observed in phytoplankton in response to a variety of stressors were also evaluated. Cell death was accompanied by significant increases in DNA degradation, intra-and extracellular ROS concentrations and DEVDase (caspase-3 like) protease activity, which have been associated with PCD in other phytoplankton species. Overall, results of this investigation provide strong evidence that treatment with the bacterial algicide, IRI-160AA results in cell cycle arrest and induces biochemical changes consistent with stress-related cell death responses observed in other phytoplankton.
Keywords:

[+]

Algal Blooms Biochemistry Dinoflagellate Blooms Shewanella Alga
Source:

Scientific Reports 7 (45102)
DOI:

https://doi.org/10.1038/srep45102
Pubmed Central ID:

PMC5362807
Document Type:

Journal Article
Funding:

Grant no. NA10NOS4780136
Rights Information:

CC BY
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PMC
Main Document Checksum:

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urn:sha256:cf69d16ea62015e5a1c2638aad4f0b1375e2e39c81dc1076cb934f097f54e639
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https://repository.library.noaa.gov/view/noaa/24065/noaa_24065_DS1.pdf
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Cell cycle arrest and biochemical changes accompanying cell death in harmful dinoflagellates following exposure to bacterial algicide IRI-160AA

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