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A surface plasmon resonance system for the underwater detection of domoic acid
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2016
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Source: Limnology and Oceanography-Methods, 14(7), 456-465.
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Journal Title:Limnology and Oceanography-Methods
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Description:Over the past decade Surface Plasmon Resonance (SPR) techniques have been applied to the measurement of numerous analytes. In this article, an SPR biosensor system deployed from an oceanographic vessel was used to measure dissolved domoic acid (DA), a common and harmful phycotoxin produced by certain microalgae species belonging to the genus Pseudo-nitzschia. During the biosensor deployment, concentrations of Pseudo-nitzschia cells were very low over the study area and measured DA concentrations were below detection. However, the in situ operational detection limit of the system was established using calibrated seawater solutions spiked with DA. The system could detect the toxin at concentrations as low as 0.1 ng mL(-1) and presented a linear dynamic range from 0.1 ng mL(-1) to 2.0 ng mL(-1). This sensor showed promise for in situ detection of DA.
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Source:Limnology and Oceanography-Methods, 14(7), 456-465.
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