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A comparison of genetic identifications and pigment patterns of Sebastes larvae caught on NOAA ship John N. Cobb cruise 9809
  • Published Date:
    2001
Filetype[PDF - 1.34 MB]


Details:
  • Corporate Authors:
    Alaska Fisheries Science Center (U.S.)
  • Series:
    AFSC processed report ; 2001-02
  • Document Type:
  • Description:
    During a cruise in Southeast Alaska in July 1998, Sebastes larvae were collected by plankton net, immediately sorted from the net, their pigment characters noted, photographed with a digital camera, and preserved for later genetic analysis. From these collections, 103 preflexion larvae had their pigment characters noted, 84 were subjected to genetic analysis (polymerase chain reaction followed by restriction fragment length analysis of the ND3/I\D4 region of mtDNA), and 67 were subjected to both pigment and genetic analysis. These larvae were grouped into eight pigment groups. The genetic analysis found seven species. With few exceptions, there was poor agreement between the pigment groups and the genetic identity of these larvae. The four larvae of Sebastes maligerlcaurinus all shared dorsal postanal melanophores (pigment group l), that were not present on any other genetically identified larvae. Pigment group 8 contained only six specimens of Sebastes zacentrus; however, the five other S. zacentrus that were identified were in four other pigment groups. Based on this study, identification to species of preflexion larvae of Sebastes from the northeast Pacific on the basis of mtDNA analysis is effective; however, identification on the basis of pigment patterns remains problematic.