Assessing the utility of AFLP for determining mutational changes in coral

Woodley, Cheryl M.; Middleton, Darlene L.; May, Lisa A. (Lisa Ann)

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Assessing the utility of AFLP for determining mutational changes in coral

2013
By Woodley, Cheryl M. ; Middleton, Darlene L. ; May, Lisa A. (Lisa Ann)

[PDF-512.80 KB]

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Personal Author:

Woodley, Cheryl M. ; Middleton, Darlene L. ; May, Lisa A. (Lisa Ann)

Woodley, Cheryl M. ; Middleton, Darlene L. ; May, Lisa A. (Lisa Ann) Less -
Corporate Authors:

United States, National Oceanic and Atmospheric Administration, ; Coral Reef Conservation Program (U.S.)

United States, National Oceanic and Atmospheric Administration, ; Coral Reef Conservation Program (U.S.) Less -
NOAA Program & Office:

CoRIS (Coral Reef Information System)
Description:

"Two variations of the AFLP technique were used in an attempt to develop an assay to screen coral for mutation accumulations. The first used the standard AFLP protocol. Results yielded small fragments <500 bp and cloning and sequencing efforts of a subset of DNA fragments showed the presence of DNA from other organisms resident in the holobiont, making this approach unsuitable for directed monitoring of coral mutational events. The second approach was a modification of the standard AFLP protocol that involved carrying out the procedure using only one restriction enzyme, Eco RI with a six-base recognition sequence. This modification did yield fragments approximately 1-1.3 kb. However, subsequent validation procedures yielded only seven coral-like DNA fragments for further analysis. Eight primer combinations of 64 possible combinations were tested. No primer combination tested amplified all samples"--Conclusions.
Keywords:

[+]

Coral Declines Coral Reef Conservation Corals Detection Effect Of Pollution On Methodology Mutation (Biology) Research Technique
Document Type:

Professional Paper
Place as Subject:

United States
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Public Domain
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Library
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urn:sha256:1ace2c3a52b5c87c2fc07128e195008611903490bc58f44ae4617dc9e39cb16c
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https://repository.library.noaa.gov/view/noaa/766/noaa_766_DS1.pdf
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Assessing the utility of AFLP for determining mutational changes in coral

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