U.S. flag An official website of the United States government.
Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

i

Analysis of 6PPD-Q in finfish, shellfish, and marine mammal tissues



Details

  • Journal Title:
    Chemosphere
  • Personal Author:
    Kuo, Li-Jung ORCID ICON IMG ; Tietsort, Jacob ORCID ICON IMG ; Bolton, Jennie L. ORCID ICON IMG ; Gates, Jonelle B. ORCID ICON IMG ; Langness, Mariko ORCID ICON IMG ; Carey, Andrea ORCID ICON IMG ; O'Neill, Sandra ORCID ICON IMG ; Schultz, Irvin R.
  • NOAA Program & Office:
    NMFS (National Marine Fisheries Service) ; NWFSC (Northwest Fisheries Science Center)
  • Description:
    6PPD-quinone (6PPD-Q), a transformation product of tire rubber anti-oxidant 6PPD, has been identified as the primary causal toxicant for the urban runoff mortality syndrome observed in coho salmon (Oncorhynchus kisutch) in the Pacific Northwest, USA. Several other fish species are also vulnerable to 6PPD-Q. However, monitoring efforts on 6PPD-Q have been focused on water, particulate matter, soils, and sediments, while that in tissues remains scarce. This study presents a workflow for extraction and quantitative analysis of 6PPD-Q in complex tissues from shellfish, finfish, and marine mammals. A multi-residue extraction protocol was developed for quantitative analysis of 6PPD-Q and persistent organic pollutants (PCBs, PBDEs, organochlorine pesticides) and PAHs in tissues in a single extraction. A GC-MS/MS based 6PPD-Q measurement was also developed. The protocol was evaluated in tissues including fish fillets, whole fish homogenates, mussels, and whale blubber. Limits of quantification of 6PPD-Q were between 0.03 and 0.12 ng/g ww and the surrogate (6PPD-Q-d5) recoveries were ∼60–100 % among matrices. We also conducted an initial biomonitoring study using caged mussels (Mytilus trossulus) and juvenile Chinook salmon (Oncorhynchus tshawytscha) from Puget Sound, WA. 6PPD-Q detection rates were at least 50 % but the concentrations were mostly <1 ng/g ww. Our protocol will aid 6PPD-Q biomonitoring in aquatic environments and also exposure assessments for improved understanding of 6PPD-Q bioaccumulation potential in these food webs.
  • Keywords:
  • Source:
    Chemosphere 379 (2025) 144418
  • DOI:
    https://doi.org/10.1016/j.chemosphere.2025.144418
  • Format:
    PDF
  • Document Type:
    Journal Article
  • Place as Subject:
    Puget Sound (Wash.)
  • License:
    https://creativecommons.org/licenses/by-nc-nd/4.0/
  • Rights Information:
    CC BY-NC-ND
  • Compliance:
    Submitted
  • Download URL:
    https://repository.library.noaa.gov/view/noaa/70809/noaa_70809_DS1.pdf
  • File Type:
    Filetype[PDF - 2.48 MB]
  • Collection(s):
    National Marine Fisheries Service (NMFS)
  • Main Document Checksum:
    urn:sha-512:d5bf44a8c324e28c0af6a1d678a9b54263058d145fe97fc1c23ace7232c6ae7304777f4f025286ddf7e664432d34c00edb6f1a31f2d25ff4a6354552c1089b32
PREVIOUS
ON THIS PAGE
Details
 Found an issue?
Send us an email at:
noaa.repository@noaa.gov
The NOAA IR serves as an archival repository of NOAA-published products including scientific findings, journal articles, guidelines, recommendations, or other information authored or co-authored by NOAA or funded partners. As a repository, the NOAA IR retains documents in their original published format to ensure public access to scientific information.
BACK TO TOP