The MIEM guidelines: Minimum information for reporting of environmental metabarcoding data

Klymus, Katy E.; Baker, Jacoby D.; Abbott, Cathryn L.; Brown, Rachel J.; Craine, Joseph M.; Gold, Zachary; Hunter, Margaret E.; Johnson, Mark D.; Jones, Devin N.; Jungbluth, Michelle J.; Jungbluth, Sean P.; Lor, Yer; Maloy, Aaron P.; Merkes, Christopher M.; Noble, Rachel; Patin, Nastassia V.; Sepulveda, Adam J.; Spear, Stephen F.; Steele, Joshua A.; Takahashi, Miwa; Watts, Alison W.; Theroux, Susanna

doi:10.3897/mbmg.8.128689

i

The MIEM guidelines: Minimum information for reporting of environmental metabarcoding data

Public Domain

2024
By Klymus, Katy E. ; Baker, Jacoby D. ; Abbott, Cathryn L. ; ...
Series: PMEL contribution no. 5641

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Journal Title:

Metabarcoding and Metagenomics
Personal Author:

Klymus, Katy E. ; Baker, Jacoby D. ; Abbott, Cathryn L. ; Brown, Rachel J. ; Craine, Joseph M. ; Gold, Zachary ; Hunter, Margaret E. ; Johnson, Mark D. ; Jones, Devin N. ; Jungbluth, Michelle J. ; Jungbluth, Sean P. ; Lor, Yer ; Maloy, Aaron P. ; Merkes, Christopher M. ; Noble, Rachel ; Patin, Nastassia V. ; Sepulveda, Adam J. ; Spear, Stephen F. ; Steele, Joshua A. ; Takahashi, Miwa ; Watts, Alison W. ; Theroux, Susanna
NOAA Program & Office:

OAR (Oceanic and Atmospheric Research) ; PMEL (Pacific Marine Environmental Laboratory)
Description:

Environmental DNA (eDNA) and RNA (eRNA) metabarcoding has become a popular tool for assessing biodiversity from environmental samples, but inconsistent documentation of methods, data and metadata makes results difficult to reproduce and synthesise. A working group of scientists have collaborated to produce a set of minimum reporting guidelines for the constituent steps of metabarcoding workflows, from the physical layout of laboratories through to data archiving. We emphasise how reporting the suite of data and metadata should adhere to findable, accessible, interoperable and reproducible (FAIR) data standards, thereby providing context for evaluating and understanding study results. An overview of the documentation considerations for each workflow step is presented and then summarised in a checklist that can accompany a published study or report. Ensuring workflows are transparent and documented is critical to reproducible research and should allow for more efficient uptake of metabarcoding data into management decision-making.
Source:

Metabarcoding and Metagenomics, 8
Series:

PMEL contribution; no. 5641
DOI:

https://doi.org/10.3897/mbmg.8.128689
ISSN:

2534-9708
Format:

PDF
Publisher:

Pensoft Publishers
Document Type:

Journal Article
License:

https://creativecommons.org/publicdomain/zero/1.0/
Rights Information:

CC0 Public Domain
Compliance:

Submitted
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urn:sha-512:7173ff15beaeb8279dfe0c307af317e0494d448fe7135f4ac43e1004e5fae575d0e7f438b408c2e0a7614c0fb4ed97fd029bc69e2a9141f4c074783e75a39377
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https://repository.library.noaa.gov/view/noaa/67456/noaa_67456_DS1.pdf
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[PDF - 1.79 MB ]

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