The NOAA IR serves as an archival repository of NOAA-published products including scientific findings, journal articles, guidelines, recommendations, or other information authored or co-authored by NOAA or funded partners.
As a repository, the NOAA IR retains documents in their original published format to ensure public access to scientific information.
i
A comparison of 4 histological staining methods for revealing oocyte development, atresia, and postovulatory follicles in 3 fish species
-
2025
-
-
Source: Fishery Bulletin, 123(1), 34-57
[PDF-24.90 MB]
Details:
-
Journal Title:Fishery Bulletin
-
Personal Author:
-
NOAA Program & Office:
-
Description:Gonad histology complements research on the life history of fish species and provides greater accuracy and precision than macroscopic characterization of the gonad for determining patterns of oogenesis at the cellular level and maturation at the individual level. In a fishery context, histology improves estimation of mature and spawning stock biomass, identification of spawning seasonality and grounds, and preselection of specimens for calculation of annual fecundity. However, in most studies, only a single staining method (hematoxylin and eosin) has been used. In this study on 3 taxonomically diverse species, using 4 different staining methods of varying complexity (degree of counterstaining), we compared confidence levels in identification of 8 stages of oogenesis, the presence and level of degradation for postovulatory follicles, and atretic (vitellogenic) germ cells. As anticipated, the method involving the least expensive, monochromatic stain provided the lowest level of confidence, whereas the most expensive and complex counterstaining method provided the highest confidence level, with hematoxylin and eosin staining and another simple counterstaining method in between them. The effect of staining method was most evident for identifying cortical alveoli, which can affect estimation of size or age at maturity, and for identifying postovulatory follicles, which can affect estimation of spawning frequency. These results are broadly applicable for determining best practices.
-
Source:Fishery Bulletin, 123(1), 34-57
-
DOI:
-
ISSN:0090-0656
-
Format:
-
Publisher:
-
Document Type:
-
License:
-
Rights Information:CC0 Public Domain
-
Compliance:Submitted
-
Main Document Checksum:
-
Download URL:
-
File Type:
