Spatial and temporal patterns of Pseudo-nitzschia genetic diversity in the North Pacific Ocean from the Continuous Plankton Recorder survey

Details

• Journal Title:
  Marine Ecology Progress Series
• Personal Author:
  Stern, R ; Moore, SK ; Trainer, VL ; Bill, BD ; Fischer, A ; Batten, S
• NOAA Program & Office:
  NMFS (National Marine Fisheries Service) ; NWFSC (Northwest Fisheries Science Center)
• Description:
  Several species of the marine diatom Pseudo-nitzschia can produce the neurotoxin domoic acid that is responsible for the seafood-borne illness amnesic shellfish poisoning in humans, marine wildlife mortalities and prolonged closures of fisheries resulting in economic losses to coastal communities. Since the year 2000, Pseudo-nitzschia species have been monitored in the Pacific Ocean with the Continuous Plankton Recorder (CPR). We used a combination of scanning electron microscopy with high-throughput and Sanger sequencing of CPR survey samples to compare the diversity of phytoplankton, including Pseudo-nitzschia species, from the north-eastern Pacific Ocean over 3 climatically different years: 2002, 2005 and 2008. A Pseudo-nitzschia-specific primer set targeting a partial region of the large subunit ribosomal DNA (rDNA) revealed spatially separated communities of Pseudo-nitzschia. The coastal region was dominated by a diverse array of P. fraudulenta unique sequences (operational taxonomic units), whereas the offshore region was rich in P. multiseries and contained a wide range of other Pseudo-nitzschia taxa, many not previously observed in this region. In 2008, exceptionally cold sea surface temperatures were recorded, influenced by a strong negative Pacific Decadal Oscillation signal. In that year, a more diverse assemblage of species was present in a spring open water sample, whereas P. fraudulenta was unusually rare from a coastal autumn sample. This is the first application of high-throughput genetic methods to uncover patterns of Pseudo-nitzschia genetic diversity from archival CPR samples, demonstrating the value of using CPR for plankton community analysis in rarely sampled regions of the oceans.
• Source:
  Marine Ecology Progress Series, 606, 7-28
• DOI:
  https://doi.org/10.3354/meps12711
• ISSN:
  0171-8630 ; 1616-1599
• Format:
  PDF
• Publisher:
  Inter-Research Science Center
• Document Type:
  Journal Article
• Rights Information:
  Other
• Compliance:
  Library
• Main Document Checksum:
  urn:sha-512:7f384a91db477039704bcc6b932b765b97c5d2782c406b3d0863e695294ebdd55d3b9e3a72dd0a18acf1a4ac87d4106deb9228e5088011a2b96bc1e2aaae4ecd
• Download URL:
  https://repository.library.noaa.gov/view/noaa/65357/noaa_65357_DS1.pdf
• File Type:
  [PDF - 1.50 MB ]