Detecting and analysing intraspecific genetic variation with eDNA: From population genetics to species abundance
Advanced Search
Select up to three search categories and corresponding keywords using the fields to the right. Refer to the Help section for more detailed instructions.

Search our Collections & Repository

For very narrow results

When looking for a specific result

Best used for discovery & interchangable words

Recommended to be used in conjunction with other fields

Dates

to

Document Data
Library
People
Clear All
Clear All

For additional assistance using the Custom Query please check out our Help Page

The NOAA IR serves as an archival repository of NOAA-published products including scientific findings, journal articles, guidelines, recommendations, or other information authored or co-authored by NOAA or funded partners. As a repository, the NOAA IR retains documents in their original published format to ensure public access to scientific information.
i

Detecting and analysing intraspecific genetic variation with eDNA: From population genetics to species abundance

Filetype[PDF-1.80 MB]


Details Supporting Files You May Also Like

Details:

  • Journal Title:
    Molecular Ecology
  • Personal Author:
  • NOAA Program & Office:
    OAR (Oceanic and Atmospheric Research)
  • Description:
    Advancements in environmental DNA (eDNA) approaches have allowed for rapid and efficient species detections in diverse environments. Although most eDNA research is focused on leveraging genetic diversity to identify taxa, some recent studies have explored the potential for these approaches to detect within‐species genetic variation, allowing for population genetic assessments and abundance estimates from environmental samples. However, we currently lack a framework outlining the key considerations specific to generating, analysing and applying eDNA data for these two purposes. Here, we discuss how various genetic markers differ with regard to genetic information and detectability in environmental samples and how analysis of eDNA samples differs from common tissue‐based analyses. We then outline how it may be possible to obtain species absolute abundance estimates from eDNA by detecting intraspecific genetic variation in mixtures of DNA under multiple scenarios. We also identify the major causes contributing to allele detection and frequency errors in eDNA data, discuss their consequences for population‐level analyses and outline bioinformatic approaches to detect and remove erroneous sequences. This review summarizes the key advances required to harness the full potential of eDNA‐based intraspecific genetic variation to inform population‐level questions in ecology, evolutionary biology and conservation management.
  • Source:
    Molecular Ecology, 32(15), 4118-4132
  • DOI:
  • ISSN:
    0962-1083;1365-294X;
  • Format:
    PDF
  • Publisher:
    Wiley
  • Document Type:
    Journal Article
  • Funding:
    Grant no. NA17OAR4320152
  • License:
  • Rights Information:
    CC BY-NC-ND
  • Compliance:
    Library
  • Main Document Checksum:
  • Download URL:
  • File Type:

You May Also Like

Checkout today's featured content at repository.library.noaa.gov

Version 3.27.1