The application of flow cytometry for kelp meiospore isolation

Augyte, Simona; Wikfors, Gary H.; Pitchford, Steve; Marty-Rivera, Michael; Umanzor, Schery; Lindell, Scott; Bailey, David; Yarish, Charles

doi:10.1016/j.algal.2020.101810

i

The application of flow cytometry for kelp meiospore isolation

2020
By Augyte, Simona ; Wikfors, Gary H. ; Pitchford, Steve ; ...

Details

Journal Title:

Algal Research
Personal Author:

Augyte, Simona ; Wikfors, Gary H. ; Pitchford, Steve ; Marty-Rivera, Michael ; Umanzor, Schery ; Lindell, Scott ; Bailey, David ; Yarish, Charles
NOAA Program & Office:

NEFSC (Northeast Fisheries Science Center) ; NMFS (National Marine Fisheries Service)
Description:

The goal of this study was to evaluate flow-cytometric techniques for isolating individual kelp meiospores into 96-welled plates. Previously reported low success rates for sorting tens of thousands of meiospores at a time have been improved by technological developments, specifically, the low nozzle pressure of the commercially available, JSAN (Bay Bioscience Co. Ltd) instrument. We monitored growth and gametophyte development post-sorting for 10 months. Our data demonstrate that successful kelp meiospore isolations of up to 76% viability can be achieved with flow-cytometry. This method can save time as compared to traditional, manual isolations using pipettes and improves confidence that self-fertilized individuals will not contaminate specific crosses of resulting gametophytes. Our results highlight a new application for the flow cytometer to produce clonal kelp gametophytes with direct applications for germplasm and culture collection development.
Keywords:

Agronomy and Crop Science
Source:

Algal Research, 46, 101810
DOI:

https://doi.org/10.1016/j.algal.2020.101810
ISSN:

2211-9264
Format:

PDF
Publisher:

Elsevier BV
Document Type:

Journal Article
Rights Information:

Accepted Manuscript
Compliance:

Library
Main Document Checksum:

urn:sha-512:3393c3445c518c1684cf19de87f410d2b49d1f3c47d2592945c3329d870deb1793b8f6a7b2baa345147cb3643458cac0f172bbdac3135c09e965e8505dd29f77
Download URL:

https://repository.library.noaa.gov/view/noaa/62534/noaa_62534_DS1.pdf
File Type:

[PDF - 563.93 KB ]

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