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Epigenetic age estimation in a long-lived, deepwater scorpionfish: insights into epigenetic clock development



Details

  • Journal Title:
    Canadian Journal of Fisheries and Aquatic Sciences
  • Personal Author:
  • NOAA Program & Office:
  • Description:
    Age estimates are essential for fisheries assessment and management, but deepwater (>200 m) fishes are often difficult to age using traditional techniques. Therefore, age-predictive epigenetic clocks were developed for a model deepwater reef fish, blackbelly rosefish Helicolenus dactylopterus, using two tissue types (fin clips and muscle; n = 61 individuals; 9−60 years) and Δ14C-validated consensus age estimates. The influence of biological information (length and sex) on epigenetic clock accuracy, and the potential for developing a multi-tissue clock, were also assessed. Bisulfite-converted restriction site-associated DNA sequencing (bsRADseq) was used to identify CpG sites (cytosines followed by guanines) exhibiting age-correlated DNA methylation, and epigenetic clocks showed strong agreement (R2 > 0.98) between predicted and consensus ages. Including length and sex data enhanced accuracy and precision (R2 > 0.99; mean absolute error < 1 year). Age-associated CpG sites were identified across tissues, but a multi-tissue clock performed poorly relative to single-tissue clocks. Overall, results demonstrate that accurate and precise epigenetic clocks can be developed for deepwater fishes, and the inclusion of biological information may enhance clock accuracy and precision.
  • Source:
    Canadian Journal of Fisheries and Aquatic Sciences (2024)
  • DOI:
  • ISSN:
    0706-652X ; 1205-7533
  • Format:
    PDF
  • Publisher:
    Canadian Science Publishing
  • Document Type:
    Journal Article
  • Funding:
  • Rights Information:
    Other
  • Compliance:
    Submitted
  • Main Document Checksum:
    urn:sha256:ad2fc4d641fc48c6ac32a8a10148d3283805b48dcc59623862f0567c8f8087b1
  • Download URL:
  • File Type:
    Filetype[PDF - 1.54 MB ]
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