Phylogenetic analysis of higher-level relationships within Hydroidolina (Cnidaria: Hydrozoa) using mitochondrial genome data and insight into their mitochondrial transcription

Kayal, Ehsan; Bentlage, Bastian; Cartwright, Paulyn; Yanagihara, Angel A.; Lindsay, Dhugal J.; Hopcroft, Russell R.; Collins, Allen G.

doi:10.7717/peerj.1403

i

Phylogenetic analysis of higher-level relationships within Hydroidolina (Cnidaria: Hydrozoa) using mitochondrial genome data and insight into their mitochondrial transcription

2015
By Kayal, Ehsan ; Bentlage, Bastian ; Cartwright, Paulyn ; ...

Details

Journal Title:

PeerJ
Personal Author:

Kayal, Ehsan ; Bentlage, Bastian ; Cartwright, Paulyn ; Yanagihara, Angel A. ; Lindsay, Dhugal J. ; Hopcroft, Russell R. ; Collins, Allen G.
NOAA Program & Office:

NMFS (National Marine Fisheries Service) ; OAR (Oceanic and Atmospheric Research) ; NEFSC (Northeast Fisheries Science Center)
Description:

Hydrozoans display the most morphological diversity within the phylum Cnidaria. While recent molecular studies have provided some insights into their evolutionary history, sister group relationships remain mostly unresolved, particularly at mid-taxonomic levels. Specifically, within Hydroidolina, the most speciose hydrozoan subclass, the relationships and sometimes integrity of orders are highly unsettled. Here we obtained the near complete mitochondrial sequence of twenty-six hydroidolinan hydrozoan species from a range of sources (DNA and RNA-seq data, long-range PCR). Our analyses confirm previous inference of the evolution of mtDNA in Hydrozoa while introducing a novel genome organization. Using RNA-seq data, we propose a mechanism for the expression of mitochondrial mRNA in Hydroidolina that can be extrapolated to the other medusozoan taxa. Phylogenetic analyses using the full set of mitochondrial gene sequences provide some insights into the order-level relationships within Hydroidolina, including siphonophores as the first diverging clade, a well-supported clade comprised of Leptothecata-Filifera III–IV, and a second clade comprised of Aplanulata-Capitatas.s.-Filifera I–II. Finally, we describe our relatively inexpensive and accessible multiplexing strategy to sequence long-range PCR amplicons that can be adapted to most high-throughput sequencing platforms.
Keywords:

General Agricultural And Biological Sciences General Biochemistry, Genetics And Molecular Biology General Medicine General Neuroscience General Agricultural And Biological Sciences General Biochemistry, Genetics And Molecular Biology General Agricultural And Biological Sciences General Biochemistry, Genetics And Molecular Biology
Source:

PeerJ, 3, e1403
DOI:

https://doi.org/10.7717/peerj.1403
ISSN:

2167-8359
Format:

PDF
Publisher:

PeerJ
Document Type:

Journal Article
Funding:

Grant no. NA08OAR4320870 ; Grant no. NA13OAR4320056
License:

https://creativecommons.org/publicdomain/zero/1.0/
Rights Information:

CC0 Public Domain
Compliance:

Library
Main Document Checksum:

urn:sha256:b9d4ac03cb4748343efd276ac73f2033e69136063556643f24bbae930c1f3b07
Download URL:

https://repository.library.noaa.gov/view/noaa/59601/noaa_59601_DS1.pdf
File Type:

[PDF - 6.78 MB ]

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