Details:

Journal Title:
Comparative Biochemistry and Physiology Part D: Genomics and Proteomics
Personal Author:
Frederick, Alyssa R. ; Heras, Joseph ; Friedman, Carolyn S. ; German, Donovan P.
Frederick, Alyssa R. ; Heras, Joseph ; Friedman, Carolyn S. ; German, Donovan P. Less -
NOAA Program & Office:
NMFS (National Marine Fisheries Service)
Description:
In the abalone and Candidatus Xenohaliotis californiensis (Ca. Xc) system, the Ca. Xc bacterium infects abalone digestive tissues and leads to extreme starvation and a characteristic “withering” of the gastropod foot. First identified in black abalone in California after an El Niño event, withering syndrome (WS) has caused large declines in wild black and captive white abalone on the northeastern Pacific coast, but disease resistance levels are species-, and possibly population-specific. This study compared gene expression patterns in the digestive gland of Ca. Xc-exposed and unexposed (control) Pinto abalone (Haliotis kamtschatkana), a particularly susceptible species. Lab-induced Ca. Xc infections were followed over 7 months and RNAseq was used to identify differential gene expression. Exposed Pinto abalone showed distinct changes in expression of 68 genes at 3 and 7 months post-infection relative to those in control animals. Upregulation of an orexin-like receptor (which is involved in feeding signaling) and a zinc peptidase-like region (many amino peptidases are zinc peptidases) in animals infected for 7 months indicates that animals with Ca. Xc infection may be starving and upregulating processes associated with feeding and digestion. Other groups of differentially expressed genes (DEGs) were upregulated or downregulated across control and exposed individuals over the 7-month experiment, including DEG groups that likely correspond to early disease state and to general stress response of being held in captivity. No patterns emerged in genes known to be involved in molluscan immune response, despite this being an expectation during a 7-month infection; digestion-related genes and unannotated DEGs were identified as targets for future research on potential immune response to WS in abalone.
Keywords:
[+]
Biochemistry Genetics Molecular Biology Physiology
Source:
Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 41, 100930
DOI:
https://doi.org/10.1016/j.cbd.2021.100930
ISSN:
1744-117X
Format:
PDF
Publisher:
Elsevier BV
Document Type:
Journal Article
Funding:
Grant no. NA14NMF4690293
License:
https://creativecommons.org/licenses/by-nc-nd/4.0/
Rights Information:
CC BY-NC-ND
Compliance:
Library
Main Document Checksum:
[+]
urn:sha256:45f0141ba88fc4d468c946ebb3f7a98976d5a45013f1b855f06da0d873dc29a7
Download URL:
https://repository.library.noaa.gov/view/noaa/59477/noaa_59477_DS1.pdf
File Type:

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