Triple oxygen stable isotope analysis of nitrite measured using continuous flow isotope ratio mass spectrometry

Details

• Journal Title:
  MethodsX
• Personal Author:
  Walters, Wendell W. ; Hastings, Meredith G.
• NOAA Program & Office:
  OAR (Oceanic and Atmospheric Research)
• Description:
  Oxygen stable isotopes (i.e., 16O, 17O, 18O) of nitrite (NO2−) are useful for investigating chemical processes and sources contributing to this important environmental contaminant and nutrient. However, it remains difficult to quantify the oxygen isotope compositions of NO2− due to the lack of internationally recognized NO2− reference materials with a well-known Δ(17O) value. Here we have adopted a combination of methodologies to develop a technique for measuring Δ(17O) of NO2− by reducing nitrate (NO3−) materials with internationally recognized Δ(17O) values to NO2− using activated cadmium catalyzed by chloride in a basic solution while conserving Δ(17O). The NO3− reference materials reduced to NO2− and sample NO2− unknowns are converted to N2O using sodium azide/acetic acid reagent and decomposed to O2 by passing through a heated gold tube and introduced into a continuous flow isotope ratio mass spectrometer for analysis at m/z 32, 33, and 34 for Δ(17O) quantification.
• Keywords:
  Clinical Biochemistry Medical Laboratory Technology
• Source:
  MethodsX, 11, 102413
• DOI:
  https://doi.org/10.1016/j.mex.2023.102413
• ISSN:
  2215-0161
• Format:
  PDF
• Publisher:
  Elsevier BV
• Document Type:
  Journal Article
• Funding:
  Grant no. NA18OAR4310118
• License:
  https://creativecommons.org/licenses/by-nc-nd/4.0/
• Rights Information:
  CC BY-NC-ND
• Compliance:
  Library
• Main Document Checksum:
  urn:sha256:5de5d83466f94b3475c1bf45f589c737c7e1add8f10ff94686b77ff442a761ed
• Download URL:
  https://repository.library.noaa.gov/view/noaa/59474/noaa_59474_DS1.pdf
• File Type:
  [PDF - 1.12 MB ]