High-Resolution Magic Angle Spinning (HRMAS) NMR Identifies Oxidative Stress and Impairment of Energy Metabolism by Zearalenone in Embryonic Stages of Zebrafish (Danio rerio), Olive Flounder (Paralichthys olivaceus) and Yellowtail Snapper (Ocyurus chrysurus)

Annunziato, Mark; Bashirova, Narmin; Eeza, Muhamed N. H.; Lawson, Ariel; Benetti, Daniel; Stieglitz, John D.; Matysik, Jörg; Alia, A.; Berry, John P.

doi:10.3390/toxins15060397

i

High-Resolution Magic Angle Spinning (HRMAS) NMR Identifies Oxidative Stress and Impairment of Energy Metabolism by Zearalenone in Embryonic Stages of Zebrafish (Danio rerio), Olive Flounder (Paralichthys olivaceus) and Yellowtail Snapper (Ocyurus chrysurus)

2023
By Annunziato, Mark ; Bashirova, Narmin ; Eeza, Muhamed N. H. ; ...

Details

Journal Title:

Toxins
Personal Author:

Annunziato, Mark ; Bashirova, Narmin ; Eeza, Muhamed N. H. ; Lawson, Ariel ; Benetti, Daniel ; Stieglitz, John D. ; Matysik, Jörg ; Alia, A. ; Berry, John P.
NOAA Program & Office:

NMFS (National Marine Fisheries Service)
Description:

Zearalenone (ZEA) is a mycotoxin, commonly found in agricultural products, linked to adverse health impacts in humans and livestock. However, less is known regarding effects on fish as both ecological receptors and economically relevant “receptors” through contamination of aquaculture feeds. In the present study, a metabolomics approach utilizing high-resolution magic angle spinning nuclear magnetic resonance (HRMAS NMR) was applied to intact embryos of zebrafish (Danio rerio), and two marine fish species, olive flounder (Paralichthys olivaceus) and yellowtail snapper (Ocyurus chrysurus), to investigate the biochemical pathways altered by ZEA exposure. Following the assessment of embryotoxicity, metabolic profiling of embryos exposed to sub-lethal concentrations showed significant overlap between the three species and, specifically, identified metabolites linked to hepatocytes, oxidative stress, membrane disruption, mitochondrial dysfunction, and impaired energy metabolism. These findings were further supported by analyses of tissue-specific production of reactive oxygen species (ROS) and lipidomics profiling and enabled an integrated model of ZEA toxicity in the early life stages of marine and freshwater fish species. The metabolic pathways and targets identified may, furthermore, serve as potential biomarkers for monitoring ZEA exposure and effects in fish in relation to ecotoxicology and aquaculture.
Keywords:

Health, Toxicology And Mutagenesis Toxicology Health, Toxicology And Mutagenesis Health, Toxicology And Mutagenesis
Source:

Toxins, 15(6), 397
DOI:

https://doi.org/10.3390/toxins15060397
ISSN:

2072-6651
Format:

PDF
Publisher:

MDPI AG
Document Type:

Journal Article
Funding:

Grant no. NA20NMF4270195
License:

https://creativecommons.org/licenses/by/4.0/
Rights Information:

CC BY
Compliance:

Library
Main Document Checksum:

urn:sha256:c553711cf63fb07e4daba25d1b1613861c4823da4cb22e865c295fcf747c872b
Download URL:

https://repository.library.noaa.gov/view/noaa/58762/noaa_58762_DS1.pdf
File Type:

[PDF - 5.98 MB ]

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