Triploid burbot, Lota lota, production: Optimization of thermal and hydrostatic parameters, tetraploid induction, and confirmation of triploid sterility

Oliver, Luke P.; Evavold, Joseph T.; Cain, Kenneth D.

doi:10.1111/jwas.13018

i

Triploid burbot, Lota lota, production: Optimization of thermal and hydrostatic parameters, tetraploid induction, and confirmation of triploid sterility

Supporting Files

2023
By Oliver, Luke P. ; Evavold, Joseph T. ; Cain, Kenneth D.

Details

Journal Title:

Journal of the World Aquaculture Society
Personal Author:

Oliver, Luke P. ; Evavold, Joseph T. ; Cain, Kenneth D.
NOAA Program & Office:

NMFS (National Marine Fisheries Service) ; NWFSC (Northwest Fisheries Science Center)
Description:

Experiments were conducted to optimize triploid induction parameters, and assess triploid sterility, in burbot. Duration and timing of shocks were based on degree minutes, temperature multiplied by time, denoted as °C min. Hydrostatic shock experiments investigated the duration of shock using 7500 or 8500 psi at 180°C min post‐fertilization. Thermal shocks investigated duration of shock and post‐fertilization shock timing using a shock of 16°C. Sterility experiments investigated egg survival when diploids were crossed with triploids. Hydrostatic shock of 7500 psi for 10 or 20°C min can induce triploidy ≥90% and exhibits survival that is statistically similar, p ≤ 0.05, to controls. Hydrostatic shock of 8500 psi for 5 or 10°C min yielded triploid induction of 93% and 100%, respectively, with survival that is statistically similar to controls, p ≤ 0.05. Thermal induction experiments indicated shocks at 120°C min post‐fertilization, for durations between 350 and 450°C min, have potential to induce triploidy ≥90% while facilitating survival statistically similar to controls, p ≤ 0.05. Induction of tetraploidy was observed. Sterility experiments determined that triploid burbot are functionally sterile. These results may allow production of burbot where sterility is required.
Keywords:

Agronomy And Crop Science Aquatic Science Agronomy And Crop Science Agronomy And Crop Science
Source:

Journal of the World Aquaculture Society (2023)
DOI:

https://doi.org/10.1111/jwas.13018
ISSN:

0893-8849 ; 1749-7345
Publisher:

Wiley
Document Type:

Journal Article
License:

https://creativecommons.org/licenses/by/4.0/
Rights Information:

CC BY
Compliance:

Library
Main Document Checksum:

urn:sha256:a1bc89cecccdd166d148b5df5efd71e85e90dfcae80ad2c5511c1add3c6f5b38
Download URL:

https://repository.library.noaa.gov/view/noaa/58292/noaa_58292_DS1.pdf
File Type:

[PDF - 644.55 KB ]

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