Acute exposure to TiO2 nanoparticles produces minimal apparent effects on oyster, Crassostrea virginica (Gmelin), hemocytes

Details

• Journal Title:
  Marine Pollution Bulletin
• Personal Author:
  Doyle, John J. ; Ward, J. Evan ; Wikfors, Gary H.
• NOAA Program & Office:
  OAR (Oceanic and Atmospheric Research)
• Description:
  The response of oyster (Crassostrea virginica) hemocytes was studied following exposure to anatase nanoparticles (ca. 7.4 nm), surface-coated rutile nanocomposites (UV–Titan M212, ca. 86 nm) and bulk titanium dioxide (TiO2) particles (anatase and rutile crystalline forms; 0.4-0.5 μm). Hemocytes were collected from oysters and exposed to one of the four particle types at concentrations of 0.1, 0.5, and 1.0 mg/L under dark and environmentally-relevant light conditions for periods of two and four hours. Hemocyte mortality, phagocytosis, and reactive oxygen species (ROS) production were then evaluated using flow-cytometric assays. Bulk and nanoparticulate TiO2 had little effect on viability of oyster hemocytes or on production of ROS. Significant changes in phagocytosis occurred after exposure to anatase nanoparticles for 4 h under dark conditions, and UV–Titan for 2 h under light conditions. Results demonstrate that TiO2 particles (bulk or nanoscale) produce minimal effects on hemocyte biomarkers examined following acute, in vitro exposures.
• Keywords:
  Aquatic Science Oceanography Pollution
• Source:
  Marine Pollution Bulletin, 127, 512-523
• DOI:
  https://doi.org/10.1016/j.marpolbul.2017.12.039
• ISSN:
  0025-326X
• Format:
  PDF
• Publisher:
  Elsevier BV
• Document Type:
  Journal Article
• Funding:
  Grant no. NA10OAR4170095
• Rights Information:
  Accepted Manuscript
• Compliance:
  Library
• Main Document Checksum:
  urn:sha256:a81e8a6f2bd6ea8f968a8361dce28f08a49a79626aeff66ae12ad3cb88c9e1f2
• Download URL:
  https://repository.library.noaa.gov/view/noaa/57891/noaa_57891_DS1.pdf
• File Type:
  [PDF - 829.81 KB ]