Real-time PCR assay for Aquimarina macrocephali subsp. homaria and its distribution in shell disease lesions of Homarus americanus, Milne-Edwards, 1837, and environmental samples

Quinn, Robert A.; Hazra, Suchandra; Smolowitz, Roxanna; Chistoserdov, Andrei Y.

doi:10.1016/j.mimet.2017.04.001

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Real-time PCR assay for Aquimarina macrocephali subsp. homaria and its distribution in shell disease lesions of Homarus americanus, Milne-Edwards, 1837, and environmental samples

2017
By Quinn, Robert A. ; Hazra, Suchandra ; Smolowitz, Roxanna ; ...
Source: Journal of Microbiological Methods, 139, 61-67

[PDF-683.55 KB]

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Journal Title:

Journal of Microbiological Methods
Personal Author:

Quinn, Robert A. ; Hazra, Suchandra ; Smolowitz, Roxanna ; Chistoserdov, Andrei Y.

Quinn, Robert A. ; Hazra, Suchandra ; Smolowitz, Roxanna ; Chistoserdov, Andrei Y. Less -
NOAA Program & Office:

NMFS (National Marine Fisheries Service)
Description:

Epizootic shell disease (ESD) is causing major losses to the lobster fishery in southern New England. Potential pathogens have been identified in lesion communities, but there are currently no efficient means of detecting and quantifying their presence. A qPCR assay was developed for a key potential pathogen, Aquimarina macrocephali subsp. homaria found to be ubiquitous in ESD lesions but not the unaffected integument. Application of the assay to various samples demonstrated that A. macrocephali subsp. homaria is ubiquitous and abundant in lobster lesions, commonly associated with healthy surfaces of crabs and is scarce in water and sediment samples from southern New England suggesting the affinity of this microorganism to the Arthropod integument. The qPCR assay developed here can be applied in future in vivo and in vitro studies to better understand the ecology and role of A. macrocephali subsp.homaria. in shell disease.
Keywords:

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Microbiology Microbiology (medical) Molecular Biology
Source:

Journal of Microbiological Methods, 139, 61-67
DOI:

https://doi.org/10.1016/j.mimet.2017.04.001
ISSN:

0167-7012
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PDF
Publisher:

Elsevier BV
Document Type:

Journal Article
Funding:

Grant no. NA06NMF4720100
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Accepted Manuscript
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Library
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urn:sha256:783e4ca064c1abab50f8bc0ea21572febbbb1910aebe82d3c54902475dd57450
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https://repository.library.noaa.gov/view/noaa/57751/noaa_57751_DS1.pdf
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Real-time PCR assay for Aquimarina macrocephali subsp. homaria and its distribution in shell disease lesions of Homarus americanus, Milne-Edwards, 1837, and environmental samples

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