Differential toxin response of Pseudo-nitzschia multiseries as a function of nitrogen speciation in batch and continuous cultures, and during a natural assemblage experiment
Advanced Search
Select up to three search categories and corresponding keywords using the fields to the right. Refer to the Help section for more detailed instructions.

Search our Collections & Repository

All these words:

For very narrow results

This exact word or phrase:

When looking for a specific result

Any of these words:

Best used for discovery & interchangable words

None of these words:

Recommended to be used in conjunction with other fields

Language:

Dates

Publication Date Range:

to

Document Data

Title:

Document Type:

Library

Collection:

Series:

People

Author:

Help
Clear All

Query Builder

Query box

Help
Clear All

For additional assistance using the Custom Query please check out our Help Page

i

Differential toxin response of Pseudo-nitzschia multiseries as a function of nitrogen speciation in batch and continuous cultures, and during a natural assemblage experiment

Filetype[PDF-1.11 MB]


Details You May Also Like

Details:

  • Journal Title:
    Harmful Algae
  • Personal Author:
  • NOAA Program & Office:
    NOAA General Documents
  • Description:
    The toxigenic diatom Pseudo-nitzschia multiseries Hasle, isolated from the U.S. Pacific Northwest, was examined in unialgal laboratory cultures and in natural assemblages during shipboard experiments, to examine cellular growth and domoic acid (DA) production as a function of nitrogen (N) substrate and availability expected during bloom development and decline. Laboratory experiments utilizing batch cultures conducted at saturating (120 μmol photons m−2 s−1) photosynthetic photon flux density (PPFD), demonstrated that P. multiseries (strain NWFSC-245) grows equally well on the three N substrates tested (nitrate [NO3−], ammonium [NH4+] and urea), and achieved an average specific growth rate of 0.83 d−1. Despite equivalent growth rates, cellular toxicity (particulate DA concentration normalized to cell abundance) varied as a function of N substrate, with urea-grown cells demonstrating 1.3- and 3.4-fold more toxicity than both NH4+- and NO3−-grown cells. Cellular toxicity of the N-limited chemostat cultures, grown at a dilution rate of 0.48 d−1, were less than the cellular toxicity measured for the N-replete batch cultures for all three N substrates, but again cellular toxicity varied as a function of N substrate and the urea-supported cells were 3.5- and 4.3-fold more toxic than the respective NH4+- and NO3−-supported cells. Starved cultures of P. multiseries showed no decline in cellular toxicity or change in the order of toxicity as a function of N substrate, and cells previously supported by urea were 13- and 5-fold more toxic than NH4+- and NO3−-supported cells. At all three levels of N-sufficiency, the urea-grown cells consistently produced the highest concentration of particulate DA per cell compared to cells grown on either NO3− or NH4+.
  • Keywords:
  • Source:
    Harmful Algae, 73, 12-29
  • DOI:
  • ISSN:
    1568-9883
  • Format:
    PDF
  • Publisher:
    Elsevier BV
  • Document Type:
    Journal Article
  • Funding:
    Grant no. NA16OP1450
  • Rights Information:
    Accepted Manuscript
  • Compliance:
    Library
  • Main Document Checksum:
  • Download URL:
  • File Type:

Supporting Files

  • No Additional Files

More +

You May Also Like

Checkout today's featured content at repository.library.noaa.gov

Version 3.26