U.S. flag An official website of the United States government.
Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

i

Molecular cloning and characterization of two ARNT (ARNT‐1 and ARNT‐2) genes in Atlantic croaker and their expression during coexposure to hypoxia and PCB77



Details

  • Journal Title:
    Environmental Toxicology
  • Personal Author:
    Rahman, Md Saydur ORCID ICON IMG ; Thomas, Peter
  • NOAA Program & Office:
    NOS (National Ocean Service)
  • Description:
    Aryl hydrocarbon receptor nuclear translocator (ARNT) is an important transcriptions factor that binds/coactivates drug‐metabolizing genes in vertebrates. In this study, we report the cloning and characterization of two ARNT (ARNT‐1 and ARNT‐2) genes and their mRNA and protein expression in liver tissues of Atlantic croaker after co‐exposure to hypoxia and 3,3′,4,4′‐tetrachlorobiphenyl (PCB77). The full‐length croaker ARNT‐1 and ARNT‐2 genes encode proteins of 537 and 530 amino acids, respectively, and are highly homologous to ARNT‐1 and ARNT‐2 genes of other vertebrates. ARNT mRNAs are ubiquitously expressed in all tissues. Hypoxia (dissolved oxygen: 1.7 mg/L) exposure (1‐4 weeks) did not affect hepatic ARNTs mRNA levels. Dietary PCB77 treatment (2 and 8 μg/g body weight/day for 4 weeks) caused marked increases in ARNTs mRNA and protein levels in normoxic fish. However, coexposure to hypoxia and PCB77 for 4 weeks significantly blunted the increase in ARNTs mRNA and protein levels in response to PCB77 exposure. These results suggest that ARNT activity and functions induced by exposure to PCB aryl hydrocarbon receptor (AhR) agonists could be compromised in croaker inhabiting hypoxic coastal regions.
  • Keywords:
  • Source:
    Environmental Toxicology, 34(2), 160-171
  • DOI:
    https://doi.org/10.1002/tox.22670
  • ISSN:
    1520-4081 ; 1522-7278
  • Format:
    PDF
  • Publisher:
    Wiley
  • Document Type:
    Journal Article
  • Funding:
    Grant no. NA09NOS4780179
  • Rights Information:
    Accepted Manuscript
  • Compliance:
    Library
  • Download URL:
    https://repository.library.noaa.gov/view/noaa/57070/noaa_57070_DS1.pdf
  • File Type:
    Filetype[PDF - 309.44 KB]
  • Collection(s):
    National Ocean Service (NOS)
  • Main Document Checksum:
    urn:sha256:b29d492114ad7e496663c0b2307449691ec43adcfaa52cc038c28fde03c0cc35
PREVIOUS
ON THIS PAGE
Details
 Found an issue?
Send us an email at:
publishinghd@cdc.gov
The NOAA IR serves as an archival repository of NOAA-published products including scientific findings, journal articles, guidelines, recommendations, or other information authored or co-authored by NOAA or funded partners. As a repository, the NOAA IR retains documents in their original published format to ensure public access to scientific information.
BACK TO TOP