Molecular cloning and characterization of two ARNT (ARNT‐1 and ARNT‐2) genes in Atlantic croaker and their expression during coexposure to hypoxia and PCB77
Advanced Search
Select up to three search categories and corresponding keywords using the fields to the right. Refer to the Help section for more detailed instructions.

Search our Collections & Repository

All these words:

For very narrow results

This exact word or phrase:

When looking for a specific result

Any of these words:

Best used for discovery & interchangable words

None of these words:

Recommended to be used in conjunction with other fields

Language:

Dates

Publication Date Range:

to

Document Data

Title:

Document Type:

Library

Collection:

Series:

People

Author:

Help
Clear All

Query Builder

Query box

Help
Clear All

For additional assistance using the Custom Query please check out our Help Page

i

Molecular cloning and characterization of two ARNT (ARNT‐1 and ARNT‐2) genes in Atlantic croaker and their expression during coexposure to hypoxia and PCB77

Filetype[PDF-309.44 KB]


Details You May Also Like

Details:

  • Journal Title:
    Environmental Toxicology
  • Personal Author:
  • NOAA Program & Office:
    NOS (National Ocean Service)
  • Description:
    Aryl hydrocarbon receptor nuclear translocator (ARNT) is an important transcriptions factor that binds/coactivates drug‐metabolizing genes in vertebrates. In this study, we report the cloning and characterization of two ARNT (ARNT‐1 and ARNT‐2) genes and their mRNA and protein expression in liver tissues of Atlantic croaker after co‐exposure to hypoxia and 3,3′,4,4′‐tetrachlorobiphenyl (PCB77). The full‐length croaker ARNT‐1 and ARNT‐2 genes encode proteins of 537 and 530 amino acids, respectively, and are highly homologous to ARNT‐1 and ARNT‐2 genes of other vertebrates. ARNT mRNAs are ubiquitously expressed in all tissues. Hypoxia (dissolved oxygen: 1.7 mg/L) exposure (1‐4 weeks) did not affect hepatic ARNTs mRNA levels. Dietary PCB77 treatment (2 and 8 μg/g body weight/day for 4 weeks) caused marked increases in ARNTs mRNA and protein levels in normoxic fish. However, coexposure to hypoxia and PCB77 for 4 weeks significantly blunted the increase in ARNTs mRNA and protein levels in response to PCB77 exposure. These results suggest that ARNT activity and functions induced by exposure to PCB aryl hydrocarbon receptor (AhR) agonists could be compromised in croaker inhabiting hypoxic coastal regions.
  • Keywords:
  • Source:
    Environmental Toxicology, 34(2), 160-171
  • DOI:
  • ISSN:
    1520-4081;1522-7278;
  • Format:
    PDF
  • Publisher:
    Wiley
  • Document Type:
    Journal Article
  • Funding:
    Grant no. NA09NOS4780179
  • Rights Information:
    Accepted Manuscript
  • Compliance:
    Library
  • Main Document Checksum:
  • Download URL:
  • File Type:

Supporting Files

  • No Additional Files

More +

You May Also Like

Checkout today's featured content at repository.library.noaa.gov

Version 3.26