An amplicon genotyping panel suitable for species identification and population genetics in sauger (Sander canadensis) and walleye (Sander vitreus)

Homola, Jared J.; Larson, Wesley A.; Albosta, Paul

doi:10.1007/s12686-023-01329-z

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An amplicon genotyping panel suitable for species identification and population genetics in sauger (Sander canadensis) and walleye (Sander vitreus)

2023
By Homola, Jared J. ; Larson, Wesley A. ; Albosta, Paul
Source: Conservation Genetics Resources (2023)

[PDF-1.18 MB]

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Journal Title:

Conservation Genetics Resources
Personal Author:

Homola, Jared J. ; Larson, Wesley A. ; Albosta, Paul

Homola, Jared J. ; Larson, Wesley A. ; Albosta, Paul Less -
NOAA Program & Office:

NMFS (National Marine Fisheries Service) ; AFSC (Alaska Fisheries Science Center)

NMFS (National Marine Fisheries Service) ; AFSC (Alaska Fisheries Science Center) Less -
Description:

Sauger (Sander canadensis) and walleye (Sander vitreus) are closely related North American fish species that are often managed by fishery agencies throughout their ranges. However, genotyping resources for sauger are presently limited to a small set of microsatellite loci. We evaluated whether primers in an existing walleye genotyping-in-thousands panel could amplify single nucleotide polymorphism loci (SNPs) in sauger. We identified 71 primer pairs that amplify 118 SNPs in both species. Allele frequency differences were large enough to confidently distinguish the species and identify hybrids. Additionally, we identified 41 loci with observed heterozygosity > 0.1 in sauger; these markers may be useful for simple population genetic analyses and parentage analysis when few contributors are present and for differentiating highly structured populations.
Keywords:

[+]

Ecology, Evolution, Behavior And Systematics Genetics
Source:

Conservation Genetics Resources (2023)
DOI:

https://doi.org/10.1007/s12686-023-01329-z
ISSN:

1877-7260
Format:

PDF
Publisher:

Springer Science and Business Media LLC
Document Type:

Journal Article
License:

https://creativecommons.org/publicdomain/zero/1.0/
Rights Information:

CC0 Public Domain
Compliance:

Submitted
Main Document Checksum:

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urn:sha256:902e38e4b215931b26a06ac3f50f05d3069dd671f6c7f1f7188453741459c98a
Download URL:

https://repository.library.noaa.gov/view/noaa/56779/noaa_56779_DS1.pdf
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An amplicon genotyping panel suitable for species identification and population genetics in sauger (Sander canadensis) and walleye (Sander vitreus)

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