Affordable de novo generation of fish mitogenomes using amplification‐free enrichment of mitochondrial DNA and deep sequencing of long fragments

Details

• Journal Title:
  Molecular Ecology Resources
• Personal Author:
  Ramón‐Laca, Ana ; Gallego, Ramón ; Nichols, Krista M.
• NOAA Program & Office:
  NMFS (National Marine Fisheries Service) ; CICOES (Cooperative Institute for Climate, Ocean and Ecosystem Studies) ; NWFSC (Northwest Fisheries Science Center)
• Description:
  Biomonitoring surveys make use of metabarcoding tools to describe the community composition. These studies match their sequencing results against public genomic databases to identify the species. However, mitochondrial genomic reference data are yet incomplete, only a few genes may be available, or the suitability of existing sequence data is suboptimal for species level resolution. Here, we present a dedicated and cost- effective workflow with no DNA amplification for generating complete fish
• Keywords:
  Biotechnology Ecology, Evolution, Behavior And Systematics Genetics Ecology, Evolution, Behavior And Systematics Ecology, Evolution, Behavior And Systematics
• Source:
  Molecular Ecology Resources, 23(4), 818-832
• DOI:
  https://doi.org/10.1111/1755-0998.13758
• ISSN:
  1755-098X ; 1755-0998
• Format:
  PDF
• Publisher:
  Wiley
• Document Type:
  Journal Article
• License:
  https://creativecommons.org/licenses/by-nc-nd/4.0/
• Rights Information:
  CC BY-NC-ND
• Compliance:
  Library
• Main Document Checksum:
  urn:sha256:4ea81656f10892f8f866a228c4d927a2a98fe63acb7de08beae629a2e194d79e
• Download URL:
  https://repository.library.noaa.gov/view/noaa/54444/noaa_54444_DS1.pdf
• File Type:
  [PDF - 2.77 MB ]