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KatharoSeq Enables High-Throughput Microbiome Analysis from Low-Biomass Samples
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2018
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Source: mSystems, 3(3)
[PDF-1.68 MB]
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Journal Title:mSystems
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Description:Various indoor, outdoor, and host-associated environments contain small quantities of microbial biomass and represent a niche that is often understudied because of technical constraints. Many studies that attempt to evaluate these low-biomass microbiome samples are riddled with erroneous results that are typically false positive signals obtained during the sampling process. We have investigated various low-biomass kits and methods to determine the limit of detection of these pipelines. Here we present KatharoSeq, a high-throughput protocol combining laboratory and bioinformatic methods that can differentiate a true positive signal in samples with as few as 50 to 500 cells. We demonstrate the application of this method in three unique low-biomass environments, including a SAF, a hospital NICU, and an abalone-rearing facility.
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Source:mSystems, 3(3)
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ISSN:2379-5077
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Rights Information:CC BY
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Compliance:Library
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