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Using Plasma Vitellogenin in Loggerhead Sea Turtles to Assess Reproductive Maturation and Estrogen‐Like Contaminant Exposure
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2023
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Source: Environmental Toxicology and Chemistry, 42(6), 1309-1325
[PDF-2.53 MB]
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Journal Title:Environmental Toxicology and Chemistry
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Description:Vitellogenin (VTG), an egg yolk precursor, is abnormally produced by male and juvenile oviparous species after exposure to estrogens. Plasma VTG in loggerhead sea turtles ( Caretta caretta ) helped us understand their reproductive maturation and investigate it as a bi omarker of contaminant exposure. The presence of VTG was screened in plasma from 404 loggerheads from the northwestern Atlantic Ocean using a freshwater turtle antibody inwestern blots. The concentra tions of VTG were semiquanti fied using band intensities calibrated to results from a log- gerhead antibody enzyme ‐linked immunoassay. The detection and conce ntrations of VTG were in (from highest to lowest): nesting females, in ‐water adult females, subadult females, smaller females, unknown sex, and males. Logger- heads from this region begin vitellogenesis at ≅77 cm straight carapace length. We classi fied VTG expression as ab-
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Source:Environmental Toxicology and Chemistry, 42(6), 1309-1325
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ISSN:0730-7268;1552-8618;
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Rights Information:CC0 Public Domain
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Compliance:Library
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