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Influence of RNA‐Seq library construction, sampling methods, and tissue harvesting time on gene expression estimation
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2023
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Source: Molecular Ecology Resources, 23(4), 803-817
[PDF-1.18 MB]
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Journal Title:Molecular Ecology Resources
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Description:RNA sequencing (RNA- Seq) is popular for measuring gene expression in non- model organisms, including wild populations. While RNA- Seq can detect gene expression variation among wild- caught individuals and yield important insights into biological function, sampling methods can also affect gene expression estimates. We examined the influence of multiple technical variables on estimated gene expression in a non- model fish, the westslope cutthroat trout ( Oncorhynchus clarkii lewisi ), using two RNA-
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Source:Molecular Ecology Resources, 23(4), 803-817
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ISSN:1755-098X;1755-0998;
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Rights Information:CC BY-NC-ND
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Compliance:Library
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