Influence of RNA‐Seq library construction, sampling methods, and tissue harvesting time on gene expression estimation

Chiari, Ylenia; Howard, Leif; Moreno, Nickolas; Relyea, Scott; Dunnigan, James; Boyer, Matthew C.; Kardos, Marty; Glaberman, Scott; Luikart, Gordon

doi:10.1111/1755-0998.13757

i

Influence of RNA‐Seq library construction, sampling methods, and tissue harvesting time on gene expression estimation

Supporting Files

2023
By Chiari, Ylenia ; Howard, Leif ; Moreno, Nickolas ; ...

Details

Journal Title:

Molecular Ecology Resources
Personal Author:

Chiari, Ylenia ; Howard, Leif ; Moreno, Nickolas ; Relyea, Scott ; Dunnigan, James ; Boyer, Matthew C. ; Kardos, Marty ; Glaberman, Scott ; Luikart, Gordon
NOAA Program & Office:

NMFS (National Marine Fisheries Service) ; NWFSC (Northwest Fisheries Science Center)
Description:

RNA sequencing (RNA- Seq) is popular for measuring gene expression in non- model organisms, including wild populations. While RNA- Seq can detect gene expression variation among wild- caught individuals and yield important insights into biological function, sampling methods can also affect gene expression estimates. We examined the influence of multiple technical variables on estimated gene expression in a non- model fish, the westslope cutthroat trout ( Oncorhynchus clarkii lewisi ), using two RNA-
Keywords:

Biotechnology Ecology, Evolution, Behavior And Systematics Genetics Ecology, Evolution, Behavior And Systematics Ecology, Evolution, Behavior And Systematics
Source:

Molecular Ecology Resources, 23(4), 803-817
DOI:

https://doi.org/10.1111/1755-0998.13757
ISSN:

1755-098X ; 1755-0998
Format:

PDF
Publisher:

Wiley
Document Type:

Journal Article
License:

https://creativecommons.org/licenses/by-nc-nd/4.0/
Rights Information:

CC BY-NC-ND
Compliance:

Library
Main Document Checksum:

urn:sha256:8aa0f1def4ddc755c70f4ea9d3e7bcea16bb1425d51acb7c51a52d2dc8f9984f
Download URL:

https://repository.library.noaa.gov/view/noaa/52846/noaa_52846_DS1.pdf
File Type:

[PDF - 1.18 MB ]

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