Details:

Journal Title:
Comparative Biochemistry and Physiology Part D: Genomics and Proteomics
Personal Author:
Wilson, Abbey E. ; Fair, Patricia A. ; Carlson, Ruth I. ; Houde, Magali ; Cattet, Marc ; ... More +
Wilson, Abbey E. ; Fair, Patricia A. ; Carlson, Ruth I. ; Houde, Magali ; Cattet, Marc ; Bossart, Gregory D. ; Houser, Dorian S. ; Janz, David M. Less -
NOAA Program & Office:
NOS (National Ocean Service) ; NCCOS (National Centers for Coastal Ocean Science)
NOS (National Ocean Service) ; NCCOS (National Centers for Coastal Ocean Science) Less -
Description:
Natural and anthropogenic stressors have been reported to impact the health of marine mammals. Therefore, investigation of quantifiable biomarkers in response to stressors is required. We hypothesized that stress protein expression would be associated with biological and health variables in wild and managed-care bottlenose dolphins (Tursiops truncatus). To test this hypothesis, our study objectives were to (1) determine if stress proteins in skin, white blood cells (WBCs), and plasma could be measured with an antibody-based microarray, (2) measure stress-protein expression relative to biological data (location, sex, age, environment), and (3) determine if stress-protein expression was associated with endocrine, hematological, biochemical and serological variables and gene expression in bottlenose dolphins. Samples were collected from two wild groups (n = 28) and two managed-care groups (n = 17). Proteins involved in the HPA axis, apoptosis, proteotoxicity, and inflammation were identified as stress proteins. The expression of 3 out of 33 proteins was significantly (P < 0.05) greater in skin than plasma and WBCs. Male dolphins had significantly greater expression levels for 10 proteins in skin compared to females. The greatest number of stress-associated proteins varied by the dolphins' environment; nine were greater in managed-care dolphins and 15 were greater in wild dolphins, which may be related to wild dolphin disease status. Protein expression in skin and WBCs showed many positive relationships with measures of plasma endocrinology and biochemistry. This study provides further understanding of the underlying mechanisms of the stress response in bottlenose dolphins and application of a combination of novel methods to measure stress in wildlife.
Keywords:
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Conservation Dolphins Tursiops Truncatus
Source:
Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 32, 100613
DOI:
https://doi.org/10.1016/j.cbd.2019.100613
ISSN:
1744-117X
Publisher:
Elsevier BV
Document Type:
Journal Article
Rights Information:
Accepted Manuscript
Rights Statement:
This manuscript is made available under the Elsevier user license https://www.elsevier.com/open-access/userlicense/1.0/
Compliance:
Library
Main Document Checksum:
[+]
urn:sha256:eaf608c19dc73b38c03448945897b4232b4c55e844e6592a09782fcd0994c324
Download URL:
https://repository.library.noaa.gov/view/noaa/50804/noaa_50804_DS1.pdf
File Type:

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