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Building genomic infrastructure: Sequencing platinum-standard reference-quality genomes of all cetacean species
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2020
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Source: Mar Mam Sci. 2020;36:1356–1366.
[PDF-819.37 KB]
Details:
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Journal Title:Marine Mammal Science
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Personal Author:
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NOAA Program & Office:
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Description:For reference-quality genomes to become a reality for all cetacean species, a globally coordinated effort among marine mammalogists is needed to obtain and preserve samples that can yield ultra-high-quality DNA and RNA, as well as the 3D genome structure for Hi-C scaffolding. Furthermore, coordination with genome centers that can perform genome sequencing, assembly, manual curation, and annotation is needed to produce reference-quality genomes and disseminate data rapidly. To begin this process, we have formed the Cetacean Genome Project (CGP) in collaboration with the VGP and Darwin Tree of Life as a coordinated effort to (1) assemble a database of samples available from accessible collections, forge collaborations and solicit appropriate samples from the scientific community; (2) coordinate and disseminate information on best practices for sample collection and preservation (e.g., cell culture, appropriate short-term field preservation methods), with facilitation of sample transportation, storage, and, where appropriate, culture of live cells; (3) coordinate available data (e.g., published short- or long-read data, genome assemblies) to avoid redundancy and reduce costs of completing the reference-quality genomes; and (4) seek funding for individual or groups of species, in coordination with marine mammal researchers with near-term interests in genomic analysis. The CGP will leverage the participation and expertise of the VGP and Darwin Tree of Life project, while providing the focus and expertise necessary to obtain samples and funding, and conduct/facilitate research on reference-quality genomes of all cetacean species. Although we have chosen to focus on a single taxonomic group, cetaceans, the issues, needs, and recommendations discussed here apply to other aquatic mammal species as well.
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Source:Mar Mam Sci. 2020;36:1356–1366.
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Rights Information:Accepted Manuscript
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Compliance:Submitted
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