ELISA methods for domoic acid quantification in multiple marine mammal species and sample matrices

Frame, Elizabeth R. (Elizabeth Rose); Lefebvre, Katherine Ann

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ELISA methods for domoic acid quantification in multiple marine mammal species and sample matrices

2013
By Frame, Elizabeth R. (Elizabeth Rose) ; Lefebvre, Katherine Ann
Series: NOAA technical memorandum NMFS-NWFSC ; 122

[PDF-153.43 KB]

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Details:

Alternative Title:

Enzyme-linked immunosorbent assay methods for domoic acid quantification in multiple marine mammal species and sample matrices
Personal Author:

Frame, Elizabeth R. (Elizabeth Rose) ; Lefebvre, Katherine Ann

Frame, Elizabeth R. (Elizabeth Rose) ; Lefebvre, Katherine Ann Less -
Corporate Authors:

Northwest Fisheries Science Center (U.S.)
NOAA Program & Office:

NMFS (National Marine Fisheries Service) ; NWFSC (Northwest Fisheries Science Center)

NMFS (National Marine Fisheries Service) ; NWFSC (Northwest Fisheries Science Center) Less -
Description:

"Over the past several years, considerable effort has been invested in developing sensitive methods for detecting algal toxins, specifically using enzyme-linked immunosorbent assay (ELISA) technologies. Previously, detection of algal toxins, such as domoic acid (DA), required expensive analytical equipment and highly trained personnel to perform high-performance liquid chromatography (HPLC) or liquid chrom atography tandem mass spectrometry (LC/MS/MS) methodologies. While excellent methods exist for DA detection using these technologies for seafood safety programs, these methods lacked the sensitivity needed for researchers investigating the impacts of harmful algal bloom toxins in wildlife. The advent of ELISA kits for DA quantification provides a useful tool for diagnostic studies in natural marine mammal populations, but requires careful testing in new sample matrices before being employed. This technical memorandum provides protocols for DA quantification by ELISA in multiple marine mammal species and sample matrices and outlines consistent reporting criteria for use by the larger scientific community"--Executive summary.
Content Notes:

Elizabeth Frame and Kathi Lefebvre.

Title from title screen (viewed on July 1, 2013).

"May 2013."

System requirements: Adobe Acrobat Reader.

Includes bibliographical references (page 15).
Keywords:

[+]

Algal Toxins California Sea Lion Domoic Acid Effect Of Water Acidification On Environmental Aspects Enzyme-linked Immunosorbent Assay Marine Mammals Marine Toxins Measurement Methodology Technique
Series:

NOAA technical memorandum NMFS-NWFSC ; 122
Document Type:

Technical Memorandum
Place as Subject:

California ; Pacific Coast

California ; Pacific Coast Less -
Rights Information:

Public Domain
Compliance:

Library
Main Document Checksum:

[+]

urn:sha256:8e786a2251b7769f65d7bcc7755a8f30c031030547e00fdbb7128de746efa616
Download URL:

https://repository.library.noaa.gov/view/noaa/4431/noaa_4431_DS1.pdf
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ELISA methods for domoic acid quantification in multiple marine mammal species and sample matrices

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