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Novel Epigenetic Age Estimation In Wild-Caught Gulf Of Mexico Reef Fishes
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2021
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Source: Canadian Journal of Fisheries and Aquatic Sciences. 79(1): 1-5.
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Journal Title:Canadian Journal of Fisheries and Aquatic Sciences
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Description:Cutting-edge DNA methylation-based epigenetic aging techniques were applied to Gulf of Mexico northern red snapper (Lutjanus campechanus n = 10 1–26 years old) and red grouper (Epinephelus morio n = 10 2–14 years old). Bisulfite-converted restriction site-associated DNA sequencing was used to identify CpG sites (cytosines followed by guanines) that exhibit age-correlated DNA methylation, and species-specific epigenetic clocks developed from hundreds of CpG sites in each species showed strong agreement between predicted and otolith-derived age (r2 > 0.99 for both species). Results suggest epigenetic age estimation could provide an accurate and efficient approach to mass-aging fishes in a non-invasive manner.
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Source:Canadian Journal of Fisheries and Aquatic Sciences. 79(1): 1-5.
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Rights Information:Accepted Manuscript
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Compliance:Submitted
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