Type Ix Secretion System Effectors And Virulence Of The Model Flavobacterium Columnare Strain Ms-Fc-4

Thunes, Nicole C.; Conrad, Rachel A.; Mohammed, Haitham H.; Zhu, Yongtao; Barbier, Paul; Evenhuis, Jason P.; Perez-Pascual, David; Ghigo, Jean-Marc; Lipscomb, Ryan S.; Schneider, John R.; Li, Nan; Erbes, Devon H.; Birkett, Clayton; Lafrentz, Benjamin R.; Welch, Timothy J.; Mcbride, Mark J.

doi:10.1128/aem.01705-21

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Type Ix Secretion System Effectors And Virulence Of The Model Flavobacterium Columnare Strain Ms-Fc-4

2022
By Thunes, Nicole C. ; Conrad, Rachel A. ; Mohammed, Haitham H. ; ...
Source: Appl Environ Microbiol. 2022 Feb 8;88(3):e0170521

[PDF-4.53 MB]

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Journal Title:

Applied and Environmental Microbiology
Personal Author:

Thunes, Nicole C. ; Conrad, Rachel A. ; Mohammed, Haitham H. ; Zhu, Yongtao ; Barbier, Paul ; ... More +

Thunes, Nicole C. ; Conrad, Rachel A. ; Mohammed, Haitham H. ; Zhu, Yongtao ; Barbier, Paul ; Evenhuis, Jason P. ; Perez-Pascual, David ; Ghigo, Jean-Marc ; Lipscomb, Ryan S. ; Schneider, John R. ; Li, Nan ; Erbes, Devon H. ; Birkett, Clayton ; Lafrentz, Benjamin R. ; Welch, Timothy J. ; Mcbride, Mark J. Less -
NOAA Program & Office:

OAR (Oceanic and Atmospheric Research) ; Sea Grant

OAR (Oceanic and Atmospheric Research) ; Sea Grant Less -
Sea Grant Program:

WISCU (Wisconsin Sea Grant)
Description:

Flavobacterium columnare causes columnaris disease in wild and cultured freshwater fish and is a major problem for sustainable aquaculture worldwide. The F. columnare type IX secretion system (T9SS) secretes many proteins and is required for virulence. The T9SS component GldN is required for secretion and gliding motility over surfaces. Genetic manipulation of F. columnare is inefficient, which has impeded identification of secreted proteins that are critical for virulence. Here, we identified a virulent wild-type F. columnare strain (MS-FC-4) that is highly amenable to genetic manipulation. This facilitated isolation and characterization of two deletion mutants lacking core components of the T9SS. Deletion of gldN disrupted protein secretion and gliding motility and eliminated virulence in zebrafish and rainbow trout. Deletion of porV disrupted secretion and virulence but not motility. Both mutants exhibited decreased extracellular proteolytic, hemolytic, and chondroitin sulfate lyase activities. They also exhibited decreased biofilm formation and decreased attachment to fish fins and other surfaces. Using genomic and proteomic approaches, we identified proteins secreted by the T9SS. We deleted 10 genes encoding secreted proteins and characterized the virulence of mutants lacking individual or multiple secreted proteins. A mutant lacking two genes encoding predicted peptidases exhibited reduced virulence in rainbow trout, and mutants lacking a predicted cytolysin showed reduced virulence in zebrafish and rainbow trout. The results establish F. columnare strain MS-FC-4 as a genetically amenable model to identify virulence factors. This may aid development of measures to control columnaris disease and impact fish health and sustainable aquaculture.
Keywords:

[+]

Diseases Fishes Flavobacterium columnare
Source:

Appl Environ Microbiol. 2022 Feb 8;88(3):e0170521
DOI:

https://doi.org/10.1128/aem.01705-21
Document Type:

Journal Article
Funding:

Grant no. NA180AR4170097
Rights Information:

Accepted Manuscript
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Submitted
Main Document Checksum:

[+]

urn:sha256:556dd773a08a99828732a53e9339cfa6c6fe0f65a3340f8e2e20978859c51f5a
Download URL:

https://repository.library.noaa.gov/view/noaa/41875/noaa_41875_DS1.pdf
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Type Ix Secretion System Effectors And Virulence Of The Model Flavobacterium Columnare Strain Ms-Fc-4

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