Metagenomic And Metatranscriptomic Insights Into Population Diversity Of Microcystis Blooms: Spatial And Temporal Dynamics Of Mcy Genotypes, Including A Partial Operon That Can Be Abundant And Expressed

Yancey, Colleen E.; Smith, Derek J.; Den Uyl, Paul A.; Mohamed, Osama G.; Yu, Fengan; Ruberg, Steven A.; Chaffin, Justin D.; Goodwin, Kelly D.; Tripathi, Ashootosh; Sherman, David H.; Dick, Gregory J.

doi:10.1128/aem.02464-21

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Metagenomic And Metatranscriptomic Insights Into Population Diversity Of Microcystis Blooms: Spatial And Temporal Dynamics Of Mcy Genotypes, Including A Partial Operon That Can Be Abundant And Expressed

2022
By Yancey, Colleen E. ; Smith, Derek J. ; Den Uyl, Paul A. ; ...
Source: AEM, Vol. 88, No. 9, 10 May 2022
Series: CIGLR contribution no. 1191

[PDF-3.78 MB]

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Details:

Journal Title:

Applied and Environmental Microbiology
Personal Author:

Yancey, Colleen E. ; Smith, Derek J. ; Den Uyl, Paul A. ; Mohamed, Osama G. ; Yu, Fengan ; ... More +

Yancey, Colleen E. ; Smith, Derek J. ; Den Uyl, Paul A. ; Mohamed, Osama G. ; Yu, Fengan ; Ruberg, Steven A. ; Chaffin, Justin D. ; Goodwin, Kelly D. ; Tripathi, Ashootosh ; Sherman, David H. ; Dick, Gregory J. Less -
NOAA Program & Office:

OAR (Oceanic and Atmospheric Research) ; AOML (Atlantic Oceanographic and Meteorological Laboratory) ; GLERL (Great Lakes Environmental Research Laboratory) ; CIGLR (Cooperative Institute for Great Lakes Research) ; Sea Grant

OAR (Oceanic and Atmospheric Research) ; AOML (Atlantic Oceanographic and Meteorological Laboratory) ; GLERL (Great Lakes Environmental Research Laboratory) ; CIGLR (Cooperative Institute for Great Lakes Research) ; Sea Grant Less -
Sea Grant Program:

OHSU (Ohio Sea Grant)
Description:

Cyanobacterial harmful algal blooms (cyanoHABs) degrade freshwater ecosystems globally. Microcystis aeruginosa often dominates cyanoHABs and produces microcystin (MC), a class of hepatotoxins that poses threats to human and animal health. Microcystin toxicity is influenced by distinct structural elements across a diversity of related molecules encoded by variant mcy operons. However, the composition and distribution of mcy operon variants in natural blooms remain poorly understood. Here, we characterized the variant composition of mcy genes in western Lake Erie Microcystis blooms from 2014 and 2018. Sampling was conducted across several spatial and temporal scales, including different bloom phases within 2014, extensive spatial coverage on the same day (2018), and frequent, autonomous sampling over a 2-week period (2018). Mapping of metagenomic and metatranscriptomic sequences to reference sequences revealed three Microcystis mcy genotypes: complete (all genes present [mcyA–J]), partial (truncated mcyA, complete mcyBC, and missing mcyD–J), and absent (no mcy genes). We also detected two different variants of mcyB that may influence the production of microcystin congeners. The relative abundance of these genotypes was correlated with pH and nitrate concentrations. Metatranscriptomic analysis revealed that partial operons were, at times, the most abundant genotype and expressed in situ, suggesting the potential biosynthesis of truncated products. Quantification of genetic divergence between genotypes suggests that the observed strains are the result of preexisting heterogeneity rather than de novo mutation during the sampling period. Overall, our results show that natural Microcystis populations contain several cooccurring mcy genotypes that dynamically shift in abundance spatiotemporally via strain succession and likely influence the observed diversity of the produced congeners.
Keywords:

[+]

Algal Blooms Fresh Water Metagenomics Lake Erie Mcy Operon Microcystis
Source:

AEM, Vol. 88, No. 9, 10 May 2022
Series:

CIGLR contribution no. 1191
DOI:

https://doi.org/10.1128/aem.02464-21
Document Type:

Journal Article
Funding:

Grant no. ; NA17OAR4320152

Grant no. ; NA17OAR4320152 Less -
Place as Subject:

Erie, Lake
Rights Information:

CC BY
Compliance:

Submitted
Main Document Checksum:

[+]

urn:sha256:605af0189f28626d14a2cbca9ff46892fa10756b124ed9bb9b94b6cc2b9e307a
Download URL:

https://repository.library.noaa.gov/view/noaa/40520/noaa_40520_DS1.pdf
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