Rescue PCR: Reagent-rich PCR recipe improves amplification of degraded DNA extracts
Advanced Search
Select up to three search categories and corresponding keywords using the fields to the right. Refer to the Help section for more detailed instructions.

Search our Collections & Repository

All these words:

For very narrow results

This exact word or phrase:

When looking for a specific result

Any of these words:

Best used for discovery & interchangable words

None of these words:

Recommended to be used in conjunction with other fields

Language:

Dates

Publication Date Range:

to

Document Data

Title:

Document Type:

Library

Collection:

Series:

People

Author:

Help
Clear All

Query Builder

Query box

Help
Clear All

For additional assistance using the Custom Query please check out our Help Page

i

Rescue PCR: Reagent-rich PCR recipe improves amplification of degraded DNA extracts

Filetype[PDF-1.18 MB]


Details You May Also Like

Details:

  • Journal Title:
    Journal of Archaeological Science: Reports
  • Personal Author:
  • NOAA Program & Office:
  • Sea Grant Program:
    WASHU (Washington Sea Grant)
  • Description:
    Minimizing the inadvertent co-extraction of polymerase chain reaction (PCR) inhibitors and/or subduing their influence are two of the most pervasive challenges in the study of ancient DNA (aDNA). Some commonly employed methods to circumvent inhibition include dilution of DNA extracts and/or removal of inhibitors via silica-based treatments. While these methods have been shown to be effective, they may not be useful for all aDNA extracts. Samples with very low copy number, for instance, may not benefit from such methods, as dilutions lower DNA concentration in tandem with the inhibitors, and some DNA loss is expected to follow silica-based treatments. Therefore, the development of additional options to overcome PCR inhibition is at a premium. In this study, we present evidence that a reagent-rich PCR protocol, where all reagents are increased in equal relative proportion can increase amplification success when DNA concentration is reduced relative to inhibitors. The reagent-rich PCR recipe, termed rescue PCR, increased amplification success by 51% for the 112 extracts used in the study. Rescue PCR represents a simple and robust addition to the suite of options currently available to work with DNA in the presence of inhibition, especially ancient, degraded, and low copy number DNA extracts.
  • Keywords:
  • Source:
    Journal of Archaeological Science: Reports 11: 683-694
  • DOI:
  • Document Type:
    Journal Article
  • Funding:
    Grant no. NA14OAR4170078
  • Rights Information:
    Accepted Manuscript
  • Compliance:
    Library
  • Main Document Checksum:
  • Download URL:
  • File Type:

Supporting Files

  • No Additional Files

More +

You May Also Like

Checkout today's featured content at repository.library.noaa.gov

Version 3.26