Exploring deep-water coral communities using environmental DNA

Everett, Meredith V.; Park, Linda K.

doi:10.1016/j.dsr2.2017.09.008

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Exploring deep-water coral communities using environmental DNA

2018
By Everett, Meredith V. ; Park, Linda K.
Source: Deep Sea Research Part II: Topical Studies in Oceanography, 150, 1-41

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Details:

Journal Title:

Deep Sea Research Part II: Topical Studies in Oceanography
Personal Author:

Everett, Meredith V. ; Park, Linda K.

Everett, Meredith V. ; Park, Linda K. Less -
NOAA Program & Office:

OAR (Oceanic and Atmospheric Research) ; Ocean Exploration Program ; NMFS (National Marine Fisheries Service) ; NWFSC (Northwest Fisheries Science Center) ; CoRIS (Coral Reef Information System)

OAR (Oceanic and Atmospheric Research) ; Ocean Exploration Program ; NMFS (National Marine Fisheries Service) ; NWFSC (Northwest Fisheries Science Center) ; CoRIS (Coral Reef Information System) Less -
Description:

Environmental DNA (eDNA) sequencing has emerged as a valuable tool for biodiversity surveys, allowing identification of taxa that may be missed by more traditional methods. Deep-sea corals, while increasingly recognized as a valuable source of habitat in the deep-ocean, have traditionally been challenging to survey. Obstacles to traditional visual surveys of these animals include the expense and complexity inherent to working in the deep marine environment, as well as the existing taxonomic uncertainty and morphological variation which can make deep-sea octocorals difficult to identify visually to the species level. This study tests an eDNA protocol for identification of deep-sea octocorals from water samples collected during the E/V Nautilus 2016 cruise season. Using this protocol, we were able to sequence eDNA from octocorals, and use these data along with image data collected during the cruise to identify taxa to the species level in a variety of habitats. eDNA sampling has the potential to complement traditional deep-sea coral surveys by overcoming the difficulty in visually identifying deep-sea octocorals and characterizing their diversity.
Keywords:

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Deep-sea Ecology Deep Sea Corals Genomics Underwater Exploration E/V Nautilus; Remotely Operated Vehicle (ROV); Deep-sea; Coral; Environmental DNA; DNA OET (Ocean Exploration Trust)
Source:

Deep Sea Research Part II: Topical Studies in Oceanography, 150, 1-41
DOI:

https://doi.org/10.1016/j.dsr2.2017.09.008
Document Type:

Journal Article
Funding:

Grant no. NA15OAR0110220 ; Grant no. NA17NOS4290190

Grant no. NA15OAR0110220 ; Grant no. NA17NOS4290190 Less -
Rights Information:

Accepted Manuscript
Compliance:

Submitted
Main Document Checksum:

[+]

urn:sha256:aac4e929cb5442c3729ffe68f9388f4ab6e4d54189b52cd06f5adf3357c92636
Download URL:

https://repository.library.noaa.gov/view/noaa/30953/noaa_30953_DS1.pdf
File Type:

	Supplemental media	zip
	Supplemental media	zip

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