Details:

Journal Title:
FEMS Microbiology Letters
Personal Author:
Bonacolta, Anthony M ; Connelly, Michael T ; M. Rosales, Stephanie ; del Campo, Javier ; Traylor-Knowles, Nikki
Bonacolta, Anthony M ; Connelly, Michael T ; M. Rosales, Stephanie ; del Campo, Javier ; Traylor-Knowles, Nikki Less -
NOAA Program & Office:
OAR (Oceanic and Atmospheric Research) ; AOML (Atlantic Oceanographic and Meteorological Laboratory) ; CIMAS (Cooperative Institute for Marine and Atmospheric Studies)
OAR (Oceanic and Atmospheric Research) ; AOML (Atlantic Oceanographic and Meteorological Laboratory) ; CIMAS (Cooperative Institute for Marine and Atmospheric Studies) Less -
Description:
Sampling of different body regions can reveal highly specialized bacterial associations within the holobiont and facilitate identification of core microbial symbionts that would otherwise be overlooked by bulk sampling methods. Here, we characterized compartment-specific associations present within the model cnidarian Nematostella vectensis by dividing its morphology into three distinct microhabitats. This sampling design allowed us to uncover a capitulum-specific dominance of spirochetes within N. vectensis. Bacteria from the family Spirochaetaceae made up 66% of the community in the capitulum, while only representing 1.2% and 0.1% of the communities in the mesenteries and physa, respectively. A phylogenetic analysis of the predominant spirochete sequence recovered from N. vectensis showed a close relation to spirochetes previously recovered from wild N. vectensis. These sequences clustered closer to the recently described genus Oceanispirochaeta, rather than Spirochaeta perfilievii, supporting them as members of this clade. This suggests a prevalent and yet uncharacterized association between N. vectensis and spirochetes from the order Spirochaetales.
Keywords:
[+]
Cnidaria Sea Anemones Spirochetes
Source:
FEMS Microbiology Letters, 368(3)
DOI:
https://doi.org/10.1093/femsle/fnab002
Document Type:
Journal Article
Rights Information:
Public Domain
Compliance:
Submitted
Main Document Checksum:
[+]
urn:sha256:85fe9500d819efa3376c6c595ff51f3ae5e5ec13e4a07a28affc40029afb6814
Download URL:
https://repository.library.noaa.gov/view/noaa/28524/noaa_28524_DS1.pdf
File Type:

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