High-resolution molecular identification of smalltooth sawfish prey

Hancock, Taylor L.; Poulakis, Gregg R.; Scharer, Rachel M.; Tolley, S. Gregory; Urakawa, Hidetoshi

doi:10.1038/s41598-019-53931-7

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The NOAA IR serves as an archival repository of NOAA-published products including scientific findings, journal articles, guidelines, recommendations, or other information authored or co-authored by NOAA or funded partners. As a repository, the NOAA IR retains documents in their original published format to ensure public access to scientific information.

i

High-resolution molecular identification of smalltooth sawfish prey

2019
By Hancock, Taylor L. ; Poulakis, Gregg R. ; Scharer, Rachel M. ; ...
Source: Sci Rep. 2019; 9: 18307.

[PDF-2.90 MB]

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Details:

Journal Title:

Scientific Reports
Personal Author:

Hancock, Taylor L. ; Poulakis, Gregg R. ; Scharer, Rachel M. ; Tolley, S. Gregory ; Urakawa, Hidetoshi

Hancock, Taylor L. ; Poulakis, Gregg R. ; Scharer, Rachel M. ; Tolley, S. Gregory ; Urakawa, Hidetoshi Less -
NOAA Program & Office:

NMFS (National Marine Fisheries Service) ; OPR (Office of Protected Resources)

NMFS (National Marine Fisheries Service) ; OPR (Office of Protected Resources) Less -
Description:

The foundation of food web analysis is a solid understanding of predator-prey associations. Traditional dietary studies of fishes have been by stomach content analysis. However, these methods are not applicable to Critically Endangered species such as the smalltooth sawfish (Pristis pectinata). Previous research using the combination of stable isotope signatures from fin clips and 18S rRNA gene sequencing of fecal samples identified the smalltooth sawfish as piscivorous at low taxonomic resolution. Here, we present a high taxonomic resolution molecular technique for identification of prey using opportunistically acquired fecal samples. To assess potential biases, primer sets of two mitochondrial genes, 12S and 16S rRNA, were used alongside 18S rRNA, which targets a wider spectrum of taxa. In total, 19 fish taxa from 7 orders and 11 families native to the Gulf of Mexico were successfully identified. The sawfish prey comprised diverse taxa, indicating that this species is a generalist piscivore. These findings and the molecular approach used will aid recovery planning for the smalltooth sawfish and have the potential to reveal previously unknown predator-prey associations from a wide range of taxa, especially rare and hard to sample species.
Keywords:

[+]

Predation (Biology) Pristis Pectinata
Source:

Sci Rep. 2019; 9: 18307.
DOI:

https://dx.doi.org/10.1038/s41598-019-53931-7
Pubmed ID:

31797939
Pubmed Central ID:

PMC6892823
Document Type:

Journal Article
Funding:

Grant no. NA10NMF4720032 ; Grant no. NA13NMF4720047

Grant no. NA10NMF4720032 ; Grant no. NA13NMF4720047 Less -
Rights Information:

CC BY
Compliance:

PMC
Main Document Checksum:

[+]

urn:sha256:0128f3cfdbd27ea57e9907ed56024e2bd813a30a140c7b6fd4157f572d7914b8
Download URL:

https://repository.library.noaa.gov/view/noaa/27594/noaa_27594_DS1.pdf
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High-resolution molecular identification of smalltooth sawfish prey

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