Achieving superresolution with illumination-enhanced sparsity

Details

• Journal Title:
  Optics Express
• Personal Author:
  Yu, Jiun-Yann ; Becker, Stephen R. ; Folberth, James ; Wallin, Bruce F. ; Chen, Simeng ; Cogswell, Carol J.
• NOAA Program & Office:
  CIRES (Cooperative Institute for Research in Environmental Sciences)
• Description:
  Recent advances in superresolution fluorescence microscopy have been limited by a belief that surpassing two-fold resolution enhancement of the Rayleigh resolution limit requires stimulated emission or the fluorophore to undergo state transitions. Here we demonstrate a new superresolution method that requires only image acquisitions with a focused illumination spot and computational post-processing. The proposed method utilizes the focused illumination spot to effectively reduce the object size and enhance the object sparsity and consequently increases the resolution and accuracy through nonlinear image post-processing. This method clearly resolves 70nm resolution test objects emitting ~530nm light with a 1.4 numerical aperture (NA) objective, and, when imaging through a 0.5NA objective, exhibits high spatial frequencies comparable to a 1.4NA widefield image, both demonstrating a resolution enhancement above two-fold of the Rayleigh resolution limit. More importantly, we examine how the resolution increases with photon numbers, and show that the more-than-two-fold enhancement is achievable with realistic photon budgets.
• Keywords:
  High Resolution Imaging Optics High Resolution Imaging
• Source:
  Optics Express 26(8): 9850-9865, 2018
• DOI:
  https://doi.org/10.1364/OE.26.009850
• Document Type:
  Journal Article
• Rights Information:
  CC BY
• Compliance:
  Submitted
• Main Document Checksum:
  urn:sha-512:2ae5db17b91e3d97817cd4c1fceec6132737428d6474c3083ae1b12335500cc9f8d15a385d68b5fc57a28441aae0d4de2920b1ab476211e4e80b4347fcea3726
• Download URL:
  https://repository.library.noaa.gov/view/noaa/27046/noaa_27046_DS1.pdf
• File Type:
  [PDF - 3.87 MB ]