Diel transcriptional response of a California Current plankton microbiome to light, low iron, and enduring viral infection

Details

• Journal Title:
  ISME Journal
• Personal Author:
  Kolody, B. C. ; McCrow, J. P. ; Allen, L. Zeigler ; Aylward, F. O. ; Fontanez, K. M. ; Moustafa, A. ; Moniruzzaman, M. ; Chavez, F. P. ; Scholin, C. A. ; Allen, E. E. ; Worden, A. Z. ; Delong, E. F. ; Allen, A. E.
• NOAA Program & Office:
  OAR (Oceanic and Atmospheric Research)
• Description:
  Phytoplankton and associated microbial communities provide organic carbon to oceanic food webs and drive ecosystem dynamics. However, capturing those dynamics is challenging. Here, an in situ, semi-Lagrangian, robotic sampler profiled pelagic microbes at 4 h intervals over ~2.6 days in North Pacific high-nutrient, low-chlorophyll waters. We report on the community structure and transcriptional dynamics of microbes in an operationally large size class (>5 μm) predominantly populated by dinoflagellates, ciliates, haptophytes, pelagophytes, diatoms, cyanobacteria (chiefly Synechococcus), prasinophytes (chiefly Ostreococcus), fungi, archaea, and proteobacteria. Apart from fungi and archaea, all groups exhibited 24-h periodicity in some transcripts, but larger portions of the transcriptome oscillated in phototrophs. Periodic photosynthesis-related transcripts exhibited a temporal cascade across the morning hours, conserved across diverse phototrophic lineages. Pronounced silica:nitrate drawdown, a high flavodoxin to ferredoxin transcript ratio, and elevated expression of other Fe-stress markers indicated Fe-limitation. Fe-stress markers peaked during a photoperiodically adaptive time window that could modulate phytoplankton response to seasonal Fe-limitation. Remarkably, we observed viruses that infect the majority of abundant taxa, often with total transcriptional activity synchronized with putative hosts. Taken together, these data reveal a microbial plankton community that is shaped by recycled production and tightly controlled by Fe-limitation and viral activity.
• Keywords:
  Phytoplankton
• Source:
  ISME J. 2019 Nov; 13(11): 2817–2833.
• DOI:
  https://doi.org/10.1038/s41396-019-0472-2
• Pubmed ID:
  31320727
• Pubmed Central ID:
  PMC6794264
• Document Type:
  Journal Article
• Funding:
  Grant no. NA15OAR4320071
• Place as Subject:
  California
• Rights Information:
  CC BY
• Compliance:
  PMC
• Main Document Checksum:
  urn:sha-512:2b235ab08280e45708037b1e76f348b40056bf07952070782890d204049a083326e788dfcc65483b863008ab30f45446ec75c6e39ca55d6613d018f6a46464b1
• Download URL:
  https://repository.library.noaa.gov/view/noaa/26323/noaa_26323_DS1.pdf
• File Type:
  [PDF - 6.74 MB ]