Standard Operating Procedures for Measuring Bulk Stable Isotope Values of Nitrogen and Carbon in Marine Biota by Isotope Ratio Mass Spectrometry (IRMS)

Gates, Jonelle B.; Chittaro, Paul; Veggerby, Karl

doi:10.25923/3mwp-ce02

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Standard Operating Procedures for Measuring Bulk Stable Isotope Values of Nitrogen and Carbon in Marine Biota by Isotope Ratio Mass Spectrometry (IRMS)

2020
By Gates, Jonelle B. ; Chittaro, Paul ; Veggerby, Karl
Series: NWFSC processed report ; 2020-04

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Personal Author:

Gates, Jonelle B. ; Chittaro, Paul ; Veggerby, Karl

Gates, Jonelle B. ; Chittaro, Paul ; Veggerby, Karl Less -
Corporate Authors:

United States. National Marine Fisheries Service ; Northwest Fisheries Science Center (U.S.)

United States. National Marine Fisheries Service ; Northwest Fisheries Science Center (U.S.) Less -
NOAA Program & Office:

NMFS (National Marine Fisheries Service) ; NWFSC (Northwest Fisheries Science Center)

NMFS (National Marine Fisheries Service) ; NWFSC (Northwest Fisheries Science Center) Less -
Description:

The isotope ratio mass spectrometer (IRMS) housed at the Northwest Fisheries Science Center (NWFSC) is used to conduct accurate and precise determinations of isotope ratios of carbon and nitrogen. NWFSC’s Environmental Chemistry Program lab facility includes a Thermo Fisher Scientific’s Flash 2000 Elemental Analyzer coupled with the Conflo IV interface and the Delta V Advantage IRMS. These systems are complex and sensitive and require detailed care and regular maintenance to ensure a high level of performance. This processed report is written to be used in conjunction with hands-on training. It is meant to be treated as a living document and will be updated as changes are made in the procedures. This report provides step-by-step instructions for sample preparation, instrument operation, maintenance of the instruments, sample archival, quality assurance (QA) and quality control (QC) measures and criteria, and data reporting.
Keywords:

[+]

Environmental Chemistry Fisheries Handbooks, Manuals, Etc. Mass Spectrometers Research
Series:

NWFSC processed report ; 2020-04
DOI:

https://doi.org/10.25923/3mwp-ce02
Document Type:

Technical Report
Place as Subject:

Northwest Coast of North America ; United States ; North Pacific Ocean

Northwest Coast of North America ; United States ; North Pacific Ocean Less -
Rights Information:

Public Domain
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Submitted
Main Document Checksum:

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urn:sha256:5597508b7427805873f9f2db7f9ab11171711604681094e8f350e426c387ee3f
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https://repository.library.noaa.gov/view/noaa/23209/noaa_23209_DS1.pdf
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Standard Operating Procedures for Measuring Bulk Stable Isotope Values of Nitrogen and Carbon in Marine Biota by Isotope Ratio Mass Spectrometry (IRMS)

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