| Growth, nutrient uptake and carbohydrate production in laboratory cultures of Spirulina major (Cyanophyceae) : final report - :13084 | Sea Grant Publications
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Growth, nutrient uptake and carbohydrate production in laboratory cultures of Spirulina major (Cyanophyceae) : final report
  • Published Date:
Filetype[PDF-1.08 MB]

  • Corporate Authors:
    Mississippi-Alabama Sea Grant Consortium ; Jackson State University ; National Sea Grant Program (U.S.)
  • Description:
    Sirulina major, a filamentous blue-green alga, has been shown under laboratory conditions to meet certain criteria necessary for its use as a potential biochemical feedstock in a wastewater/ aquaculture system. The adaptability of S. major to various percentages of wastewater/ seawater mixtures enhances its use in estuarine environments. Optimal growth conditions for S. major in the laboratory have been demonstrated to be, a temperature of 30 C, low light intensity, salinity range of 4-30 ppt, and a pH range of 8.5-10.5. Cultures have been observed to remain relatively free of contamination if the pH is kept within the range and the wastewater is membrane filtered. Biomass productions of 130 mg dry wt/liter/day of S. major were obtained. Carbohydrate content of cells increased from 16.4% in cells in exponential growth to 42.2% in cells subjected to low nitrogen conditions. Under laboratory conditions, reductions of eutrophicating nutrients were found to be approximately 97% for NH4, 100% for NO3 and 47% for PO4. The attached growth habit of S. major allows for ease of harvest. Glass has been demonstrated to be a good substrate in providing additional surface area for growth and harvesting. Additions of human urine (0.1-0.5%) under laboratory conditions appears as good as domestic/commercial wastewater as a useable source of nutrients to generate algal biomass.

  • Document Type:
  • Funding:
    Funding: NOAA Office of Sea Grant; grant number: NA81AA-D-00050; project number: R/MT-4;
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