Details:

Journal Title:
Environmental Health Perspectives
Personal Author:
Temkin, Alexis M. ; Bowers, Robert R. ; Magaletta, Margaret E. ; Holshouser, Steven ; Maggi, Adriana ; ... More +
Temkin, Alexis M. ; Bowers, Robert R. ; Magaletta, Margaret E. ; Holshouser, Steven ; Maggi, Adriana ; Ciana, Paolo ; Guillette, Louis J. ; Bowden, John A. ; Kucklick, John R. ; Baatz, John E. ; Spyropoulos, Demetri D. Less -
NOAA Program & Office:
NOS (National Ocean Service) ; DWH (Deepwater Horizon)
NOS (National Ocean Service) ; DWH (Deepwater Horizon) Less -
Description:
BACKGROUND: The obesity pandemic is associated with multiple major health concerns. In addition to diet and lifestyle, there is increasing evidence that environmental exposures to chemicals known as obesogens also may promote obesity. OBJECTIVES: We investigated the massive environmental contamination resulting from the Deepwater Horizon (DWH) oil spill, including the use of the oil dispersant COREXIT in remediation efforts, to determine whether obesogens were released into the environment during this incident. We also sought to improve the sensitivity of obesogen detection methods in order to guide post-toxicological chemical assessments. METHODS: Peroxisome proliferator-activated receptor gamma (PPAR.) transactivation assays were used to identify putative obesogens. Solid-phase extraction (SPE) was used to sub-fractionate the water-accommodated fraction generated by mixing COREXIT, cell culture media, and DWH oil (CWAF). Liquid chromatography-mass spectrometry (LC-MS) was used to identify components of fractionated CWAF. PPAR response element (PPRE) activity was measured in PPRE-luciferase transgenic mice. Ligand-binding assays were used to quantitate ligand affinity. Murine 3T3-L1 preadipocytes were used to assess adipogenic induction. RESULTS: Serum-free conditions greatly enhanced the sensitivity of PPAR. transactivation assays. CWAF and COREXIT had significant dose-dependent PPAR. transactivation activities. From SPE, the 50: 50 water: ethanol volume fraction of CWAF contained this activity, and LC-MS indicated that major components of COREXIT contribute to PPAR. transactivation in the CWAF. Molecular modeling predicted several components of COREXIT might be PPAR. ligands. We classified dioctyl sodium sulfosuccinate (DOSS), a major component of COREXIT, as a probable obesogen by PPAR. transactivation assays, PPAR-driven luciferase induction in vivo, PPAR. binding assays (affinity comparable to pioglitazone and arachidonic acid), and in vitro murine adipocyte differentiation. CONCLUSIONS: We conclude that DOSS is a putative obesogen worthy of further study, including epidemiological and clinical investigations into laxative prescriptions consisting of DOSS.
Source:
Environmental Health Perspectives, 124(1), 112-119.
DOI:
https://doi.org/10.1289/ehp.1409672
Document Type:
Journal Article
Rights Information:
CC BY
Compliance:
Submitted
Main Document Checksum:
[+]
urn:sha256:c3735992ffa877be730e9f84cf8c912e33995d312316fd30aaaf2282e9f8f668
Download URL:
https://repository.library.noaa.gov/view/noaa/13740/noaa_13740_DS1.pdf
File Type:

Supporting Files

• ehp.1409672.alt.pdf

  pdf

More +