Advanced Search
Select up to three search categories and corresponding keywords using the fields to the right. Refer to the Help section for more detailed instructions.

Search our Collections & Repository

All these words:

For very narrow results

This exact word or phrase:

When looking for a specific result

Any of these words:

Best used for discovery & interchangable words

None of these words:

Recommended to be used in conjunction with other fields

Language:

Dates

Publication Date Range:

to

Document Data

Title:

Document Type:

Library

Collection:

Series:

People

Author:

Help
Clear All

Query Builder

Query box

Help
Clear All

For additional assistance using the Custom Query please check out our Help Page

i

Integrating DNA barcoding of fish eggs into ichthyoplankton monitoring programs

Filetype[PDF-2.03 MB]


Details You May Also Like

Details:

  • Journal Title:
    Fishery Bulletin
  • Personal Author:
  • NOAA Program & Office:
    NMFS (National Marine Fisheries Service)
  • Description:
    The data collected through ichthyoplankton monitoring surveys provide valuable insight into the spawning dynamics of multiple species. Fish eggs, more than larvae, offer a more precise evaluation of species-specific spawning characteristics; however, egg collections are greatly underused because of the limitations associated with morphology-based identifications. In recent years, a new means of molecular identification, termed DNA barcoding, has made species identification readily available across a broad range of taxa. We used DNA barcoding to identify ethanol-preserved fish eggs collected during 2002-2012 along the northeastern U.S. continental shelf A subsampling protocol was used to select 1603 unidentified eggs for analysis. DNA sequences were successfully obtained from 1495 (93.26%) of these eggs, representing 50 species many of which have either never before been identified to the species-level as eggs or have been identified previously only to a higher taxonomic level or during specific developmental egg stages. In comparison with past attempts at morphological identification, our molecular identifications comprise a broader diversity of eggs and provide a technique with high success rates of unambiguous identifications that is not sensitive to egg stage. Overall, this work shows that DNA barcoding of fish eggs is sufficiently advanced to be incorporated into long-term, regional-scale ichthyoplankton. monitoring programs.
  • Source:
    Fishery Bulletin, 114(2), 153-165.
  • DOI:
  • Document Type:
    Journal Article
  • Rights Information:
    CC BY
  • Compliance:
    Submitted
  • Main Document Checksum:
  • Download URL:
  • File Type:

Supporting Files

  • No Additional Files

More +

You May Also Like

Checkout today's featured content at repository.library.noaa.gov

Version 3.26